Culture-Independent Analysis of Fecal Microbiota in Cattle

DOI Web Site Web Site PubMed 被引用文献7件
  • OZUTSUMI Yuhei
    United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center
  • HAYASHI Hidenori
    Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center
  • SAKAMOTO Mitsuo
    Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center
  • ITABASHI Hisao
    United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology
  • BENNO Yoshimi
    Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center

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The phylogenetic diversity of the fecal bacterial community in Holstein cattle was determined by 16S ribosomal RNA gene sequence analysis. The sequences were affiliated with the following phyla: Firmicutes (81.3%), Bacteroidetes (14.4%), Actinobacteria (2.5%), and Proteobacteria (1.4%). The Clostridium leptum subgroup was the most phylogenetically diverse group in cattle feces. In addition, a number of previously uncharacterized and unidentified bacteria were recognized in clone libraries.

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