Functional Conservation between Fission Yeast moc1/sds23 and Its Two Orthologs, Budding Yeast SDS23 and SDS24, and Phenotypic Differences in Their Disruptants

• GOLDAR Md. Muniruzzaman

  Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University

• NISHIE Toshikazu

  Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University

• ISHIKURA Yasue

  Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University

• FUKUDA Toshifumi

  Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University

• TAKEGAWA Kaoru

  Department of Life Sciences, Faculty of Agriculture, Kagawa University

• KAWAMUKAI Makoto

  Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University

Bibliographic Information

Other Title

• Functional Conservation between Fission Yeast<i>moc1/sds23</i>and Its Two Orthologs, Budding Yeast<i>SDS23</i>and<i>SDS24</i>, and Phenotypic Differences in Their Disruptants

Abstract

The moc1/sds23 gene was isolated to induce sexual development of a sterile strain due to overexpression of adenylate cyclase in Schizosaccharomyces pombe. Here, we studied the functional conservation between moc1/sds23 and its two orthologs SDS23 and SDS24 in Saccharomyces cerevisiae. We observed that the temperature sensitivity, salt tolerance, cell morphology, and sterility of the Δmoc1 mutant in S. pombe were recovered by expressing either S. cerevisiae SDS23 or SDS24. We found that deletion of both SDS23 and SDS24 resulted in the production of a large vacuole that was reversed by the expression of S. pombe moc1/sds23. In these ways we found that S. pombe Moc1/Sds23 and S. cerevisiae SDS23p or SDS24p are functional homologs. In addition we found that the Δsds23 Δsds24 diploid strain reduces cell separation in forming pseudohyphal-like growth in S. cerevisiae. Thus S. pombe moc1/sds23 and S. cerevisiae SDS23 or SDS24 are interchangeable with each other, but their disruptants are phenotypically dissimilar.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 69 (7), 1422-1426, 2005

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• <I>Schizosaccharomyces pombe</I>
• <I>Saccharomyces cerevisiae</I>
• <I>moc1</I>
• vacuole
• pseudohyphae

Details

• CRID: 1390282681451044992
• NII Article ID: 10016644222
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.69.1422
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 7378597
• PubMed: 16041152
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7378597
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.69.1422
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed