Two Tandemly Arranged Ferredoxin Genes in the<i>Hydrogenobacter thermophilus</i>Genome: Comparative Characterization of the Recombinant [4Fe–4S] Ferredoxins

• IKEDA Takeshi

  Department of Biotechnology, University of Tokyo

• YAMAMOTO Masahiro

  Department of Biotechnology, University of Tokyo

• ARAI Hiroyuki

  Department of Biotechnology, University of Tokyo

• OHMORI Daijiro

  Department of Chemistry, School of Medicine, Juntendo University

• ISHII Masaharu

  Department of Biotechnology, University of Tokyo

• IGARASHI Yasuo

  Department of Biotechnology, University of Tokyo

書誌事項

タイトル別名

• Two Tandemly Arranged Ferredoxin Genes in the Hydrogenobacter thermophilus Genome: Comparative Characterization of the Recombinant [4Fe-4S] Ferredoxins

抄録

A thermophilic, obligately chemolithoautotrophic hydrogen-oxidizing bacterium, Hydrogenobacter thermophilus TK-6, assimilates carbon dioxide via the reductive tricarboxylic acid cycle. A gene encoding a ferredoxin involved in this cycle as an electron donor (HtFd1) was cloned and sequenced. Interestingly, another ferredoxin gene (encoding HtFd2) was found in tandem with the HtFd1 gene. These two ferredoxin genes overlapped by four bp, and transcriptional analysis revealed that they are co-transcribed as an operon. The deduced amino acid sequences of HtFd1 and HtFd2 were 42.9% identical and each contained four cysteine residues that serve as probable ligands to an iron-sulfur cluster. Spectroscopic analyses of the purified recombinant ferredoxins heterologously expressed in Escherichia coli indicated that each ferredoxin contains a single [4Fe–4S]^2+⁄1+ cluster.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 69 (6), 1172-1177, 2005

  公益社団法人 日本農芸化学会

キーワード

• <I>Hydrogenobacter thermophilus</I>
• <I>Aquifex aeolicus</I>
• ferredoxin
• operon
• reductive tricarboxylic acid cycle

詳細情報

• CRID: 1390282681449681024
• NII論文ID: 130000030303
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.69.1172
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 7349733
• PubMed: 15973049
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7349733
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.69.1172
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可