Thermostability of Refolded Ovalbumin and S-Ovalbumin

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Abstract

Ovalbumin, a member of the serpin superfamily, is transformed into a thermostabilized form, <I>S</I>-ovalbumin, during storage of shell eggs or by an alkaline treatment of the isolated protein (Δ<I>T<SUB>m</SUB></I>=8 °C). As structural characteristics of <I>S</I>-ovalbumin, three serine residues (Ser164, Ser236 and Ser320) take the <small>D</small>-amino acid residue configuration, while the conformational change from non-thermostabilized native ovalbumin is very small (Yamasaki, M., Takahashi, N., and Hirose, M., <I>J. Biol. Chem.</I>, <B>278</B>, 35524–35530 (2003)). To assess the role of the structural characteristics on protein thermostabilization, ovalbumin and <I>S</I>-ovalbumin were denatured to eliminate the conformational modulation effects and then refolded. The denatured ovalbumin and <I>S</I>-ovalbumin were correctly refolded into the original non-denatured forms with the corresponding differential thermostability. There was essentially no difference in the disulfide structures of the native and refolded forms of ovalbumin and <I>S</I>-ovalbumin. These data are consistent with the view that the configuration inversion, which is the only chemical modification directly detected in <I>S</I>-ovalbumin so far, plays a central role in ovalbumin thermostabilization. The rate of refolding of <I>S</I>-ovalbumin was greater than that of ovalbumin, indicating the participation, at least in part, of an increased folding rate for thermodynamic stabilization.

Journal

  • Bioscience, Biotechnology, and Biochemistry

    Bioscience, Biotechnology, and Biochemistry 69(5), 922-931, 2005-05-23

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

References:  39

Codes

  • NII Article ID (NAID)
    130000030322
  • NII NACSIS-CAT ID (NCID)
    AA10824164
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    09168451
  • NDL Article ID
    7322398
  • NDL Source Classification
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL Call No.
    Z53-G223
  • Data Source
    CJP  NDL  J-STAGE 
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