Effects of Serum Deprivation on Expression of Proteolytic-Related Genes in Chick Myotube Cultures

• NAKASHIMA Kazuki

  Nutrient Function Laboratory, Department of Animal Physiology and Nutrition, National Institute of Livestock and Grassland Science

• YAMAZAKI Makoto

  Nutrient Function Laboratory, Department of Animal Physiology and Nutrition, National Institute of Livestock and Grassland Science

• ABE Hiroyuki

  Nutrient Function Laboratory, Department of Animal Physiology and Nutrition, National Institute of Livestock and Grassland Science

抄録

We previously reported that serum deprivation stimulates myofibrillar proteolysis in chick myotubes. In the present study, we examined the effect of serum deprivation on expression of the proteolytic-related genes (ubiquitin, proteasome, calpains, and cathepsin B) by real-time PCR of cDNA in chick myotubes. Myotubes were incubated with serum-free medium for 24 h. Ubiquitin and proteasome subunits (C1 and C2) and calpains (m-, μ-, and p94/calpain-3) but not cathepsin B mRNA expression were increased by serum deprivation. These results indicate that serum deprivation stimulates ubiquitin-proteasome and calpain proteolytic pathways, resulting in an increase in myofibrillar proteolysis in chick myotubes.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 69 (3), 623-627, 2005

  公益社団法人 日本農芸化学会

キーワード

• myotubes
• calpain
• ubiquitin
• proteasome
• serum deprivation

詳細情報

• CRID: 1390282681450669824
• NII論文ID: 130000030403
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.69.623
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 7295590
• PubMed: 15784993
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7295590
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.69.623
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可