Growth Inhibition by Tungsten in the Sulfur-Oxidizing Bacterium<i>Acidithiobacillus thiooxidans</i>

• NEGISHI Atsunori

  Technical Research Institute, Hazama Corporation

• MURAOKA Tadashi

  Division of Science and Technology for Energy Conversion, Graduate School of Natural Science and Technology, Okayama University

• MAEDA Terunobu

  Civil Chemical Engineering Corporation

• TAKEUCHI Fumiaki

  Environmental Management and Safety Section, Health and Environment Center, Okayama University

• KANAO Tadayoshi

  Faculty of Agriculture, Okayama University

• KAMIMURA Kazuo

  Faculty of Agriculture, Okayama University

• SUGIO Tsuyoshi

  Division of Science and Technology for Energy Conversion, Graduate School of Natural Science and Technology, Okayama University

書誌事項

タイトル別名

• Growth Inhibition by Tungsten in the Sulfur-Oxidizing Bacterium Acidithiobacillus thiooxidans

抄録

Growth of five strains of sulfur-oxidizing bacteria Acidithiobacillus thiooxidans, including strain NB1-3, was inhibited completely by 50 μM of sodium tungstate (Na₂WO₄). When the cells of NB1-3 were incubated in 0.1 M β-alanine–SO₄²⁻ buffer (pH 3.0) with 100 μM Na₂WO₄ for 1 h, the amount of tungsten bound to the cells was 33 μg/mg protein. Approximately 10 times more tungsten was bound to the cells at pH 3.0 than at pH 7.0. The tungsten binding to NB1-3 cells was inhibited by oxyanions such as sodium molybdenum and ammonium vanadate. The activities of enzymes involved in elemental sulfur oxidation of NB1-3 cells such as sulfur oxidase, sulfur dioxygenase, and sulfite oxidase were strongly inhibited by Na₂WO₄. These results indicate that tungsten binds to NB1-3 cells and inhibits the sulfur oxidation enzyme system of the cells, and as a result, inhibits cell growth. When portland cement bars supplemented with 0.075% metal nickel and with 0.075% metal nickel and 0.075% calcium tungstate were exposed to the atmosphere of a sewage treatment plant containing 28 ppm of H₂S for 2 years, the weight loss of the portland cement bar with metal nickel and calcium tungstate was much lower than the cement bar containing 0.075% metal nickel.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 69 (11), 2073-2080, 2005

  公益社団法人 日本農芸化学会

キーワード

• tungsten
• <I>Acidithiobacillus thiooxidans</I>
• concrete corrosion
• bacteriostatic agent
• sulfur oxidation

詳細情報

• CRID: 1390282681449488256
• NII論文ID: 130000030479
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.69.2073
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 7728818
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7728818
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.69.2073
• 本文言語コード: en
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