Interaction between Trehalose and Alkaline-Earth Metal Ions

• OKU Kazuyuki

  Amase Institute, Hayashibara Biochemical Laboratories, Inc.

• KUROSE Mayumi

  Amase Institute, Hayashibara Biochemical Laboratories, Inc.

• KUBOTA Michio

  Amase Institute, Hayashibara Biochemical Laboratories, Inc.

• FUKUDA Shigeharu

  Amase Institute, Hayashibara Biochemical Laboratories, Inc.

• KURIMOTO Masashi

  Amase Institute, Hayashibara Biochemical Laboratories, Inc.

• TUJISAKA Yoshio

  Amase Institute, Hayashibara Biochemical Laboratories, Inc.

• SAKURAI Minoru

  Center for Biological Resources and Informatics, Tokyo Institute of Technology

Abstract

We investigated the interaction between trehalose and alkaline-earth metal ions. The nuclear relaxation times of carbon atoms of trehalose were shortened by addition of the alkaline-earth chloride salts, MgCl₂, CaCl₂, and SrCl₂, indicating that trehalose formed metal-complexes with the alkaline-earth metal chlorides. From the data of the ¹H–¹H coupling constants of trehalose in the presence of the alkaline-earth chlorides, it appeared that trehalose formed complexes with MgCl₂, and CaCl₂ at the various complexing sites: Mg²⁺ was coordinated to O-4 and O-4′ of trehalose, and Ca²⁺ to O-2 and O-3. We succeeded in the preparation of two types of crystals of the trehalose/CaCl₂. One was a crystal consisting of trehalose, CaCl₂, and water in a ratio of 1:1:1. The other was an anhydrous crystal containing trehalose and CaCl₂ in a ratio of 1:2. Several applications of the complexing between trehalose and the metal ions for food processing are proposed.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 69 (1), 7-12, 2005

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• trehalose
• alkaline-earth metal
• trehalose/calcium chloride complex
• chlorophyll
• insoluble matter (<I>aku</I>)

Details

• CRID: 1390282681453279872
• NII Article ID: 130000030546
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.69.7
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 7237072
• PubMed: 15665460
• Web Site: http://id.ndl.go.jp/bib/7237072
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7237072
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.69.7
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed