Occurrence of Fructosyl-Amino Acid Oxidase-Reactive Compounds in Fungal Cells

  • YOSHIDA Nobuyuki
    Graduate School of Biological Sciences, Nara Institute of Science and Technology
  • TAKATSUKA Kenji
    Graduate School of Biological Sciences, Nara Institute of Science and Technology
  • KATSURAGI Tohoru
    Graduate School of Biological Sciences, Nara Institute of Science and Technology
  • TANI Yoshiki
    Graduate School of Biological Sciences, Nara Institute of Science and Technology

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Fructosyl-amino acid oxidase (FAOD)-reactive fraction (FRY) was found in commercial yeast extract. FRY showed very hydrophilic property and was adsorbed to phenylboronate silica gel, indicating that it contained the Amadori compound. TLC and amino acid analyses revealed that glucosone, lysine, and arginine were produced from FRY after incubation with FAOD. TOF-MS analysis confirmed that FRY is a mixture of fructosyl lysine and fructosyl arginine. These compounds were also detected in mycelial extract of an FAOD-producer, Aspergillus terreus GP1, grown on the minimum medium, suggesting that a glycation reaction occurs in fungal cells and that FAOD acts toward the resultant Amadori compounds.

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