Effect of a Pore-Forming Protein Derived from Flammulina velutipes on the Caco-2 Intestinal Epithelial Cell Monolayer

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Author(s)

Abstract

We have previously found a transepithelial electrical resistance (TEER)-decreasing protein derived from <I>Flammulina velutipes</I>, which was revealed to be identical to flammutoxin (FTX) that is known as a hemolytic pore-forming protein. This protein induced a rapid decrease in TEER and parallel increase in paracellular permeability in the intestinal epithelial Caco-2 cell monolayer without any cytotoxicity. An immunoblotting analysis revealed that the FTX-induced decrease in TEER was accompanied by the formation of a high-molecular-weight complex on the surface of Caco-2 cells. Intracellular Ca<SUP>2+</SUP> imaging showed that exposure to FTX caused a rapid Ca<SUP>2+</SUP> influx. It was observed by electron microscopy that FTX induced swelling of microvilli and expansion of the cellular surface. Staining with fluorescent phalloidin showed a marked change to filamentous actin in the FTX-treated cells.<BR>These results suggest that TEER reduction could sensitively detect small membrane pore formation by FTX in the intestinal epithelium which causes a morphological alteration and disruption of the paracellular barrier function.

Journal

  • Bioscience, Biotechnology, and Biochemistry

    Bioscience, Biotechnology, and Biochemistry 68(11), 2230-2238, 2004-11-23

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

References:  36

Cited by:  1

Codes

  • NII Article ID (NAID)
    130000030909
  • NII NACSIS-CAT ID (NCID)
    AA10824164
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    09168451
  • NDL Article ID
    7173152
  • NDL Source Classification
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL Call No.
    Z53-G223
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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