Successful Mouse Cloning of an Outbred Strain by Trichostatin A Treatment after Somatic Nuclear Transfer
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- KISHIGAMI Satoshi
- Center for Developmental Biology, RIKEN Kobe
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- BUI Hong-Thuy
- Center for Developmental Biology, RIKEN Kobe
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- WAKAYAMA Sayaka
- Center for Developmental Biology, RIKEN Kobe
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- TOKUNAGA Kenzo
- Department of Pathology, National Institute of Infectious Diseases
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- THUAN Nguyen Van
- Center for Developmental Biology, RIKEN Kobe
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- HIKICHI Takafusa
- Center for Developmental Biology, RIKEN Kobe
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- MIZUTANI Eiji
- Center for Developmental Biology, RIKEN Kobe
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- OHTA Hiroshi
- Center for Developmental Biology, RIKEN Kobe
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- SUETSUGU Rinako
- Center for Developmental Biology, RIKEN Kobe
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- SATA Tetsutaro
- Department of Pathology, National Institute of Infectious Diseases
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- WAKAYAMA Teruhiko
- Center for Developmental Biology, RIKEN Kobe
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抄録
Although the somatic cloning technique has been used for numerous applications and basic research of reprogramming in various species, extremely low success rates have plagued this technique for a decade. Further in mice, the "clonable" strains have been limited to mainly hybrid F1 strains such as B6D2F1. Recently, we established a new efficient cloning technique using trichostatin A (TSA) which leads to a 2-5 fold increase in success rates for mouse cloning of B6D2F1 cumulus cells. To further test the validity of this TSA cloning technique, we tried to clone the adult ICR mouse, an outbred strain, which has never been directly cloned before. Only when TSA was used did we obtain both male and female cloned mice from cumulus and fibroblast cells of adult ICR mice with 4-5% success rates, which is comparable to 5-7% of B6D2F1. Thus, the TSA treatment is the first cloning technique to allow us to successfully clone outbred mice, demonstrating that this technique not only improves the success rates of cloning from hybrid strains, but also enables mouse cloning from normally "unclonable" strains.<br>
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 53 (1), 165-170, 2007
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282681314004480
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- NII論文ID
- 130000053946
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- NII書誌ID
- AA10936678
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- COI
- 1:STN:280:DC%2BD2s7jtVCksg%3D%3D
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 8653327
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- PubMed
- 17077581
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可