Determination of Glycarbylamide Based on Formation of Nickel Chelate

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  • TAJIMA Noriko
    Showa University, School of Pharmaceutical Sciences
  • MIZUNO Yasuharu
    National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries
  • HAMAMOTO Kouko
    National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries
  • ARAKAWA Hidetoshi
    Showa University, School of Pharmaceutical Sciences
  • MAEDA Masako
    Showa University, School of Pharmaceutical Sciences

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Other Title
  • ニッケルキレート生成を利用したグリカルピラミドの分析法
  • ニッケルキレート セイセイ オ リヨウ シタ グリカルピラミド ノ ブンセキホウ
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Abstract

A new and simple analytical method for glycarbylamide (GB) based on the formation of nickel chelate was developed. The proposed method is as follows: sample solution is mixed with 0.08 mmol/L nickel nitrate in 0.2 mol/L carbonate buffer (pH 9.0), and the absorbance is measured at 290 nm. Under the optimal conditions, GB could be determined in the concentration range from 0.13 μg/mL to 2.6 μg/mL (r=0.9999). Using this method for HPLC post column reaction, GB levels in chicken liver extracts could be determined. The recovery of GB was 79.4% (RSD=2.6%, n=3) and the quantitation limit was 30 ng/g. The apparent molar extinction coefficient (ε) of the GB-nickel complex was 8.5×103. The molar ratio of the complex is GB : nickel ion=2 : 1.

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