PCR法を用いた飼料中の魚由来DNAの検出法の検討  [in Japanese] Detection of Fish DNA in Ruminant Feed by PCR Amplification  [in Japanese]

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Abstract

わが国において,魚介類由来タンパク質を牛用飼料に使用することが禁止されたため,著者らは通知法「PCRによる飼料中の動物由来DNAの検出法」と同様なPCR法を用いる公定試験法を開発検討した.魚粉にはさまざまな種類の魚が使われるため,魚のミトコンドリアDNAから共通な領域を探し,魚以外の動植物が混在する飼料から魚由来DNAだけを特異的に検出できるプライマーを設計した.著者らが既報で示したプライマーではウシ用配合飼料に微量混入した魚粉を検出することができなかったが,本分析法では配合飼料に0.01~0.001%含まれる魚粉を検出することができた.

The Japanese Government has prohibited the use of seafood protein, as well as mammalian protein, in ruminant feed. There is an official method to detect meat and bone meal, but no method is yet available to detect fishmeal in ruminant feed. We tried to develop a suitable method to detect fishmeal in ruminant feed, similar to the official method “PCR detection of animal-derived DNA in feed”. Our previously reported primers (fishcon5 and fishcon3-1) showed low sensitivity, so we designed new primers based on a DNA sequence from yellowfin tuna mitchondrial DNA. Among the primers, FM5 and FM3 specifically detected fish DNA (sardine, yellowfin tuna, skipjack tuna, chub mackerel, Pacific saury, salmon, rainbow trout, Japanese anchovy, codfish and Japanese horse mackerel) from fish meat, and did not amplify DNA from animals and plants. The sensitivity for detection of the presence of fishmeal in ruminant feed was 0.01-0.001%.

Journal

  • Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)

    Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 47(5), 222-224, 2006

    Japanese Society for Food Hygiene and Safety

Codes

  • NII Article ID (NAID)
    130000054189
  • NII NACSIS-CAT ID (NCID)
    AN00117741
  • Text Lang
    JPN
  • Article Type
    journal article
  • ISSN
    0015-6426
  • Data Source
    J-STAGE  JASI 
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