cDNAマイクロアレイによるウシ栄養膜細胞株(BT-1)の遺伝子発現プロファイル

DOI 日本農学文献記事索引 Web Site Web Site PubMed 参考文献58件
  • USHIZAWA Koichi
    Reproductive Biology and Technology Laboratory, Developmental Biology Department, National Institute of Agrobiological Sciences
  • TAKAHASHI Toru
    Reproductive Biology and Technology Laboratory, Developmental Biology Department, National Institute of Agrobiological Sciences
  • KANEYAMA Kanako
    Reproductive Biology and Technology Laboratory, Developmental Biology Department, National Institute of Agrobiological Sciences
  • TOKUNAGA Tomoyuki
    Development and Differentiation Laboratory, Developmental Biology Department, National Institute of Agrobiological Sciences
  • TSUNODA Yukio
    Laboratory of Animal Reproduction, College of Agriculture, Kinki University
  • HASHIZUME Kazuyoshi
    Reproductive Biology and Technology Laboratory, Developmental Biology Department, National Institute of Agrobiological Sciences Department of Veterinary Medicine, Faculty of Agriculture, Iwate University

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タイトル別名
  • Gene Expression Profiles of Bovine Trophoblastic Cell Line (BT-1) Analyzed by a Custom cDNA Microarray

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Gene expression of bovine trophoblast cell line (BT-1) was analyzed with a custom utero-placenta complementary DNA microarray. Expression comparison with in vivo tissues of trophoblast derivation was performed to investigate characteristics of the expression in BT-1. BT-1 is a cell line established without feeder cells using trophoblast cells that are separated from bovine blastocysts. The bovine in vivo tissues of the trophoblast derivation were collected on day 17 through 56 of gestation. Of 1,773 genes, 933 exhibited an expression difference exceeding two-fold between BT-1 and in vivo tissues. These genes were analyzed by the k-means clustering method and were distributed into six clusters. Some genes, such as placental lactogen, interferon-τ and pregnancy-associated glycoproteins, all known as trophoblast-specific genes, were detected in BT-1 as well as in vivo tissues throughout the experiment period. These trophoblast-specific genes and octamer-binding transcription factor-4, known as a marker for undifferentiation of cells in mice, were detected by RT-PCR in both BT-1 and in vivo trophoblast tissues. The overall gene expression profile in BT-1 suggests that this cell line contains trophoblast-specific characteristics and is similar to trophoblast cells around the implantation period.<br>

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