Effect of Relaxin on Acrosome Reaction and Utilization of Glucose in Boar Spermatozoa

• MIAH A.G.

  Laboratory of Animal Biotechnology, Interdisciplinary Graduate School of Science and Technology, Shinshu University

• TAREQ K.M.A.

  Laboratory of Animal Biotechnology, Interdisciplinary Graduate School of Science and Technology, Shinshu University

• HAMANO Ko-ichi

  Laboratory of Animal Biotechnology, Interdisciplinary Graduate School of Science and Technology, Shinshu University

• KOHSAKA Tetsuya

  Laboratory of Animal Reproduction, Faculty of Agriculture, Shizuoka University

• TSUJII Hirotada

  Laboratory of Animal Biotechnology, Interdisciplinary Graduate School of Science and Technology, Shinshu University

抄録

Relaxin is a peptide hormone found in seminal plasma that has a physiological influence on sperm motility in some species. There are no reports on the effect of relaxin on acrosome reaction and utilization of glucose in boar spermatozoa. In this study, to investigate the effects of relaxin on sperm motility, acrosome reaction, and incorporation and oxidation of labeled glucose, boar spermatozoa were washed and preincubated for swim-up and then incubated (0-6 h) with 0, 20, or 40 ng/ml relaxin in mTALP medium. The results indicated that the addition of relaxin stimulated sperm motility significantly (P<0.05) during 1-4 h of incubation. The percentage of acrosome-reacted live spermatozoa was higher (P<0.05) when the spermatozoa were treated with 20 or 40 ng/ml relaxin. The rate of incorporation, and oxidation of glucose were also greater (P<0.05) in the spermatozoa incubated with relaxin compared to the control spermatozoa. The rate of incorporation and oxidation of ¹⁴C-glucose were increased in correlation with acrosome reaction up to 4 h of incubation and then decreased in line with the increasing incubation period. In conclusion, the present study demonstrates that relaxin accelerates not only motility but also the acrosome reaction and utilization of glucose in boar spermatozoa.<br>

収録刊行物

• Journal of Reproduction and Development

  Journal of Reproduction and Development 52 (6), 773-779, 2006

  公益社団法人 日本繁殖生物学会

キーワード

• Acrosome reaction
• Boar spermatozoa
• Relaxin
• Sperm motility
• Utilization of glucose

詳細情報

• CRID: 1390282681314963968
• NII論文ID: 130000054583
• NII書誌ID: AA10936678
• DOI: 10.1262/jrd.18037
• ISSN: 13484400
• ISSN: 09168818
• NDL書誌ID: 8596700
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8596700
• Web Site: http://www.jstage.jst.go.jp/article/jrd/52/6/52_6_773/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2007202025
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • CiNii Articles
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• 抄録ライセンスフラグ: 使用不可