マウス乳頭・乳腺組織におけるプロラクチン遺伝子発現の解析

DOI 日本農学文献記事索引 Web Site Web Site PubMed 被引用文献1件 参考文献59件
  • KOIZUMI Minoru
    Laboratory of Functional Anatomy, Department of Life Sciences, Faculty of Agriculture, Meiji University Laboratory of Molecular Biology and Gene Regulation, Department of Life Sciences, Faculty of Agriculture, Meiji University
  • HORIGUCHI Kotaro
    Laboratory of Functional Anatomy, Department of Life Sciences, Faculty of Agriculture, Meiji University
  • TOMITA Yoko
    Laboratory of Functional Anatomy, Department of Life Sciences, Faculty of Agriculture, Meiji University
  • KATO Yukio
    Laboratory of Molecular Biology and Gene Regulation, Department of Life Sciences, Faculty of Agriculture, Meiji University
  • HARIGAYA Toshio
    Laboratory of Functional Anatomy, Department of Life Sciences, Faculty of Agriculture, Meiji University

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タイトル別名
  • Prolactin Gene Expression in the Mouse Nipple

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In this study, we attempted to examine the presence of prolactin (PRL) messenger ribonucleic acid (mRNA) and protein in the mouse nipple and mammary gland in pregnancy and lactation. PRL-like substances were found by immunohistochemistry using an antibody against the mouse PRL (mPRL) in the sebaceous gland cells of the nipple during late pregnancy and lactation, and the cistern of alveoli in mammary glands during lactation. Western blot analysis of proteins extracted from the nipple and the mammary gland showed immunoreactive bands corresponding to molecular weights of approximate 16 kDa and 32 kDa, respectively. The expression of mRNA for mPRL in the nipple and mammary gland during late pregnancy and lactation was demonstrated by reverse transcription-polymerase chain reaction (RT-PCR), Southern blotting, and nucleotide sequence analyses. These results suggest that mPRL mRNA and its translation product are synthesized in the mouse nipple.

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