Factors Affecting Premature Chromosome Condensation of Cumulus Cell Nuclei Injected Into Rat Oocytes
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- HIRABAYASHI Masumi
- National Institute for Physiological Sciences
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- KATO Megumi
- National Institute for Physiological Sciences CREST of Japan Science and Technology Corporation
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- TAKEUCHI Ayumu
- Faculty of Textile Science and Technology, Shinshu University
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- ISHIKAWA Ayako
- National Institute for Physiological Sciences
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- HOCHI Shinichi
- Faculty of Textile Science and Technology, Shinshu University
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To date, production of cloned rats by somatic cell nuclear transfer (NT) has not yet been successful. Inducing premature chromosome condensation (PCC) of injected cell nuclei in recipient cytoplasm is considered essential for successful mouse cloning by the Honolulu method. In the present study, some factors affecting PCC of rat cumulus cell nuclei injected into rat oocytes were examined. Wistar female rats (young: 4 to 5-week-old, mature: ≥10-week-old) were superovulated by injections of eCG and hCG, and oocytes recovered 14 or 17 h after hCG injection were received with cumulus cell nuclei using piezo-driven micromanipulator. When the oocytes were recovered 14 h post-hCG injection from young rats and the nuclear injection into oocytes was completed within 45 min, PCC was observed in 44-49% of NT oocytes. In the case of oocytes from mature rats, PCC occurred in 11-19% of the NT oocytes. Oocytes recovered 17 h post-hCG injection did not support PCC of the injected nuclei (0-7%) regardless of the donor age. Treatment of oocytes with a neutral cysteine protease inhibitor, N-acetylleucylleucylnorleucinal, slightly increased the incidence of PCC (48 vs 37%). Comparison of rat strains for oocyte donors indicated that proportions of NT oocytes undergoing PCC in Wistar and LEW oocytes (41-46%) were higher than those in Donryu and F344 oocytes (17-25%). Thus, ability of rat oocytes to promote PCC of the injected nuclei is dependent on the characteristics of oocytes, such as age or strain of donor rats, and timing of oocyte recovery.
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 49 (2), 121-126, 2003
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282681314396416
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- NII論文ID
- 10026618752
- 130000054896
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- NII書誌ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 6514762
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- PubMed
- 14967936
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 使用不可