Analysis of correlation between nuclear DNA content, chromosome number, and flowering capacity of asymmetric somatic hybrids of diploid Solanum brevidens and (di)haploid S. tuberosum.

• VALKONEN J. P. T.

  Department of Plant Pathology

• WATANABE K. N.

  Department of Plant Breeding and Biometry, Cornell University

• PEHU E.

  Department of Plant Production

書誌事項

タイトル別名

• Analysis of correlation between nuclear

抄録

Selected stable standard Solanum genotypes and two populations of asymmetric somatic hybrids between Solanum brevidens (2n=2x=24) and dihaploid S. tuberosum (2n=2x=24) were analyzed to test the resolution of flow cytometric determination of nuclear DNA content (2C value) in terms of a loss or gain of chromosomes, and to analyze the correlation between the 2C value and the chromosome number. Correlation was high within the standard genotypes (r=0.99, P<0.001). However, the correlation was low within the two populations of somatic hybrids (r=0.54 and 0.65, 0.01<P<0.05). Highly chimeric , hybrids were readily detected by flow cytometry, whereas microscopic chromosome observations failed to detect them. Data were analyzed regarding their potential usefulness in predicting the flowering capacity. The 2C values deviating from those of the euploid standard genotypes were highly associated with abnormal morphology and lack of flowering. Flowering capacity did not correlate with chromosome number. These results suggest that flow cytometry can be utilized for initial selection of somatic hybrids.<br>

収録刊行物

• 遺伝学雑誌

  遺伝学雑誌 69 (5), 525-536, 1994

  日本遺伝学会

キーワード

• Genetics
• General Medicine

詳細情報

• CRID: 1390001205443598848
• NII論文ID: 130000056800
• NII書誌ID: AA00690924
• DOI: 10.1266/jjg.69.525
• ISSN: 18805787
• ISSN: 0021504X
• NDL書誌ID: 3908784
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3908784
• Web Site: http://www.jstage.jst.go.jp/article/jjg/69/5/69_5_525/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可