Enin Isolated from Skin of Red Grape(Vitris vinifera) Induces Apoptosis in Human Lymphoid Leukemia Molt 4B Cells.

• MOTEKI Hiroyuki

  International Flavors & Fragrances (Japan) Ltd.

• HIBASAMI Hiroshige

  Faculty of Medicine, Mie University

• OHWAKI Shinji

  Faculty of Bioresource, Mie University

• KATSUZAKI Hirotaka

  Faculty of Bioresource, Mie University

• IMAI Kunio

  Faculty of Bioresource, Mie University

• ISHII Yaeko

  Faculty of Medicine, Mie University

• NAKAGAWA Masako

  Faculty of Medicine, Mie University

• KOMIYA Takashi

  Faculty of Bioresource, Mie University

抄録

We have investigated the effect of extract prepared from skin of red grape (Vitris vinifera) on the growth of human lymphoid leukemia Molt 4B cells. It was found that the extract obtained by extraction with butanol suppressed the growth of Molt 4B cells. Fraction IV obtained by separation with HPLC inhibited the growth of the Molt 4B cells by 86% at the concentration of 600 μM. We determined the structure of compound contained in Fraction IV and identified it as enin, a kind of anthocyanin, by ¹H- and ¹³C-NMR. The enin-treated cells clearly exhibited morphological changes, indicating the blebbing and chromatin condensation which are characteristic of apoptosis. Characteristic oligonucleosomal-sized DNA fragments were observed in the enin-treated cells. These findings suggest that the suppression by enin of growth of Molt 4B cells results from the induction of apoptosis by this compound. <br>

収録刊行物

• Food Science and Technology Research

  Food Science and Technology Research 7 (2), 131-134, 2001

  公益社団法人 日本食品科学工学会

キーワード

• enin
• growth inhibition
• apoptosis
• leukemia
• skin of red grape (<i>Vitris vinifera</i>)

詳細情報

• CRID: 1390001204455195008
• NII論文ID: 130000061709
• NII書誌ID: AA11320122
• DOI: 10.3136/fstr.7.131
• ISSN: 18813984
• ISSN: 13446606
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可