Nonsynonymous Site Heteroplasmy in Fish Mitochondrial DNA

• Shigenobu Yuya

  Department of Fisheries Science, Kitasato University

• Saitoh Kenji

  Tohoku National Fisheries Research Institute

• Hayashizaki Ken-ichi

  Department of Fisheries Science, Kitasato University

• Ida Hitoshi

  Department of Fisheries Science, Kitasato University

Abstract

Heteroplasmic nucleotide polymorphisms are rarely observed in wild animal mitochondrial DNA. The occurrence of such site heteroplasmy is expected to be extremely rare at nonsynonymous sites where the number of nucleotide substitutions per site is low due to functional constraints. This report deals with nonsynonymous mitochondrial heteroplasmy from two wild fish species, chum salmon and Japanese flounder. We detected an A/C nonsynonymous heteroplasmic site corresponding to putative amino acids, Ile or Met, in NADH dehydrogenase subunit-5 (ND5) region of chum salmon. The heteroplasmic site was at the 3rd position of 58th codon. As for Japanese flounder we detected a C/T nonsynonymous heteroplasmic site corresponding to putative amino acids, Leu or Pro, in ND4 region. The heteroplasmic site was at the 2nd position of 450th codon. We also verified heteroplasmy at these sites by sequencing cloned fragments.<br>

Journal

• Genes & Genetic Systems

  Genes & Genetic Systems 80 (4), 297-301, 2005

  The Genetics Society of Japan

Keywords

• <b>Key words: </b>wild animal
• nucleotide substitution
• codon position
• <i>Oncorhynchus keta</i>
• <i>Paralichthys olivaceus</i>

Details

• CRID: 1390001205472441856
• NII Article ID: 130000062183
• NII Book ID: AA11077421
• DOI: 10.1266/ggs.80.297
• ISSN: 18805779
• ISSN: 13417568
• NDL BIB ID: 7681548
• Web Site: http://id.ndl.go.jp/bib/7681548
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7681548
• Web Site: http://www.jstage.jst.go.jp/article/ggs/80/4/80_4_297/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed