Rapid construction of <italic>Drosophila</italic> RNAi transgenes using pRISE, a P-element-mediated transformation vector exploiting an in vitro recombination system.
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- Kondo Takefumi
- Graduate School of Biological Sciences, Nara Institute of Science and Technology
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- Inagaki Sachi
- Graduate School of Biological Sciences, Nara Institute of Science and Technology
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- Yasuda Kunio
- Graduate School of Biological Sciences, Nara Institute of Science and Technology
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- Kageyama Yuji
- Graduate School of Biological Sciences, Nara Institute of Science and Technology
書誌事項
- タイトル別名
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- Rapid construction of Drosophila RNAi transgenes using pRISE, a P-element-mediated transformation vector exploiting an in vitro recombination system
- Rapid construction of Drosophila RNAi transgenes using pRIZE, a P-element-mediated transformation vector exploiting an in vitro recombination system
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抄録
RNAi is a gene-silencing phenomenon mediated by double-stranded RNA (dsRNA) and has become a powerful tool to elucidate gene function. To accomplish rapid construction of transgenes expressing dsRNA in Drosophila, we developed a novel transformation vector, pRISE, which contains an inverted repeat of the attR1-ccdB-attR2 cassette for in vitro recombination and a pentameric GAL4 binding site for conditional expression. These features enabled us to construct RNAi transgenes without a complicated cloning scheme. In cultured cells and transgenic flies, pRISE constructs carrying dsRNA transgenes induced effective RNAi against an EGFP transgene and the endogenous white gene, respectively. These results indicate that pRISE is a convenient transformation vector for studies of multiple Drosophila genes for which functional information is lacking.<br>
収録刊行物
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- Genes & Genetic Systems
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Genes & Genetic Systems 81 (2), 129-134, 2006
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詳細情報 詳細情報について
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- CRID
- 1390001205473092864
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- NII論文ID
- 130000062210
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- NII書誌ID
- AA11077421
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- ISSN
- 18805779
- 13417568
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- NDL書誌ID
- 7933177
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可