Construction of tobamovirus vector that can systemically spread and express foreign gene products in Solanaceous plants
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- HORI Koichi
- Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo
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- WATANABE Yuichiro
- Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo
書誌事項
- タイトル別名
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- Construction of a Tobamovirus Vector That Can Systemically Spread and Express Foreign Gene Products in Solanaceous Plants.
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抄録
In present-day biology, a facile gene delivery system is required to study gene function in a high throughput way. The use of plant virus vectors that can introduce foreign or extra endogenous genes into plants attracts much attention. It is advantageous in that researchers need only minimal experience and results can be obtained in a short period of time. Here we describe infectious cDNA clones of the tomato mosaic tobamoviruses (ToMV) that were modified for facile insertion of foreign genes and which retained the ability to multiply in plants. This vector, named TocJ, was able to harbor the GFP gene from Aequorea victoria and systemically spread in some Solanaceous plants. TocJ would be suitable for the overexpression of foreign genes and the analysis of gene functions in various Solanaceous plants.
収録刊行物
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- Plant Biotechnology
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Plant Biotechnology 20 (2), 129-136, 2003
日本植物バイオテクノロジー学会
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詳細情報 詳細情報について
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- CRID
- 1390282679305200512
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- NII論文ID
- 130000064157
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- NII書誌ID
- AA11250821
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- COI
- 1:CAS:528:DC%2BD3sXksFSqsL4%3D
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- ISSN
- 13476114
- 13424580
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- NDL書誌ID
- 6545527
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
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- 抄録ライセンスフラグ
- 使用不可