Development of Apoptosis and Changes in Apoptosis-related Genes Expression in the Mouse Thymus Following T-2 Toxin-inoculation.

• Shinozuka Junko

  Department of Veterinary Pathology, Faculty of Agriculture, The University of Tokyo

• Tsutsui Shigeki

  Department of Biomedical Science, Faculty of Agriculture, The University of Tokyo

• Ishigami Noriaki

  Department of Veterinary Pathology, Faculty of Agriculture, The University of Tokyo

• Ueno-Yamanouchi Aito

  Department of Veterinary Pathology, Faculty of Agriculture, The University of Tokyo

• Nakayama Hiroyuki

  Department of Veterinary Pathology, Faculty of Agriculture, The University of Tokyo

• Doi Kunio

  Department of Veterinary Pathology, Faculty of Agriculture, The University of Tokyo

抄録

T-2 toxin is a kind of trichothecene mycotoxin produced by species of the genus Fusarium, and it attacks the tissues containing a large number of actively dividing cells such as lymphoid and hematopoietic tissues. The development of apoptosis and the changes in mRNA expressions of apoptosis-related cell-surface antigen (Fas) and genes (p53, bcl-2, c-myc, and c-fos) were investigated in the thymus up to 9 hours after oral inoculation (HAI) with 10 mg/kg body weight of T-2 toxin in female ICR: CD-1 mice. The number of TUNEL-positive lymphocytes clearly increased in the thymus of T-2 toxin-inoculated mice at 6 and 9 HAI. By agarose gel electrophoresis, DNA ladder was clearly detected at 9 HAI. The c-fos mRNA expression increased prior to the progression of apoptotic cell death in the thymus of T-2 toxin-inoculated mice. As to Fas, p53, bcl-2, and c-myc, their mRNA expressions showed no significant changes through the observation period. The results using p53 knockout mice and Fas disfunctional mutant mice also demonstrated that p53 and Fas had no relation to T-2 toxin-induced apoptosis. These findings suggest that c-fos mRNA induction may be associated with T-2 toxin-induced lymphocyte apoptosis.

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ｔｏｘｉｃｏｌｏｇｉｃ　Ｐａｔｈｏｌｏｇｙ

  Ｊｏｕｒｎａｌ　ｏｆ　Ｔｏｘｉｃｏｌｏｇｉｃ　Ｐａｔｈｏｌｏｇｙ 12 (2), 77-81, 1999

  日本毒性病理学会

キーワード

• T-2 toxin
• Apoptosis
• Lymphocytes
• <i>c-fos</i>
• Mouse

詳細情報

• CRID: 1390001204414502784
• NII論文ID: 130000064846
• NII書誌ID: AA12022786
• DOI: 10.1293/tox.12.77
• COI: 1:CAS:528:DyaK1MXmsVKjs7s%3D
• ISSN: 1881915X
• ISSN: 09149198
• Web Site: http://www.jstage.jst.go.jp/article/tox/12/2/12_2_77/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2000001875
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可