国動協および公私動協において動物実験に用いられたサルおよびブタのHEV感染調査 Serological Evidence for Hepatitis E Virus Infection in Laboratory Monkeys and Pigs in Animal Facilities in Japan

Access this Article

Author(s)

    • KUROSAWA Tsutomu
    • Institute of Experimental Animal Sciences, Osaka University Medical School
    • SHIBAHARA Toshiyuki
    • Division of Laboratory Animal Science, Research Center for Bioscience and Technology, Tottori University
    • FURUYA Masato
    • Division of Laboratory Animal Science, Section of Life Science and Bio Functional Materials, Science Research Center, Kochi University
    • MOHRI Shirou
    • Center of Biomedical Research, Facility of Medical Sciences, Kyushu University
    • SATO Hiroshi
    • Division of Comparative Medicine, Center for Frontier Life Sciences, Nagasaki University
    • OHSAWA Kazutaka
    • Division of Comparative Medicine, Center for Frontier Life Sciences, Nagasaki University
    • IBUKI Kentaro
    • Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University
    • TAKEDA Naokazu
    • Department of Virology II, National Institute of Infectious Diseases
    • LI Tian-Cheng
    • Department of Virology II, National Institute of Infectious Diseases
    • KOSHIMOTO Chihiro
    • Frontier Science Research Center, Bio-resource Division, University of Miyazaki
    • ITO Kaori
    • Division for Animal Resources and Development, Life Science Research Center, University of Toyama
    • KITA Masakazu
    • Department of Microbiology, Kyoto Prefectural University of Medicine
    • MIYASHITA Nobumoto
    • Division of Animal Experimentation, Life Science Research Center, Kagawa University
    • ARIKAWA Jiro
    • Institute for Animal Experimentation, Hokkaido University Graduate School of Medicine
    • ASANO Masahide
    • Division of Transgenic Animal Science, Advanced Science Research Center, Kanazawa University

Abstract

In laboratory animal facilities, monkeys and pigs are used for animal experiments, but the details of hepatitis E virus (HEV) infection in these animals are unknown. The risk of infection from laboratory animals to humans has become a concern; therefore, much attention should be paid to the handling of these animals during their care and use, including surgical procedures performed on infected animals. In this connection, serum samples collected from 916 monkeys and 77 pigs kept in 23 animal facilities belonging to the Japanese Association of Laboratory Animal Facilities of National University Corporations (JALAN) and the Japanese Association of Laboratory Animal Facilities of Public and Private Universities (JALAP) in Japan were examined for the purpose of detecting antibodies to HEV and HEV RNA by using ELISA and RT-PCR, respectively. One hundred and seven serum samples of 916 (11.7%) monkeys were positive for anti-HEV IgG, and 7 and 17 serum samples of 916 (0.8% and 5.3%) monkeys were positive for anti-HEV IgM and IgA, respectively. Thirty-six samples from 62 (58.1%) farm pigs were positive for anti-HEV IgG, whereas all samples tested from miniature pigs were negative (0/15, 0%). Seven samples from 62 (9.1%) farm pigs and 7 samples from 916 (0.8%) monkeys were positive for IgM antibody, but these HEV-IgM antibody positive serum samples were HEV-RNA negative by RT-PCR. The IgM antibody positive rate (9.1%) of farm pigs was much higher than that of monkeys (0.8%). These results suggest the relative levels of risk of HEV infection from these animals to animal handlers and researchers who work with them in laboratory animal facilities.<br>

Journal

  • Experimental Animals

    Experimental Animals 57(4), 367-376, 2008

    Japanese Association for Laboratory Animal Science

Codes

  • NII Article ID (NAID)
    130000067977
  • NII NACSIS-CAT ID (NCID)
    AA11032321
  • Text Lang
    ENG
  • ISSN
    1341-1357
  • NDL Article ID
    9566050
  • NDL Source Classification
    ZS7(科学技術--医学)
  • NDL Call No.
    Z54-H752
  • Data Source
    NDL  J-STAGE 
Page Top