Establishment of immortalized clonal cells derived from periodontal ligament cells by induction of the hTERT gene

• Fujita Tomohisa

  Department of Dentistry for the Disabled, Nihon University School of Dentistry at Matsudo

• Otsuka-Tanaka Yoko

  Department of Dentistry for the Disabled, Nihon University School of Dentistry at Matsudo Department of Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo

• Tahara Hidetoshi

  Department of Cellular and Molecular Biology, Hiroshima University Faculty of Medicine

• Ide Toshinori

  Department of Cellular and Molecular Biology, Hiroshima University Faculty of Medicine

• Abiko Yoshimitsu

  Department of Biochemistry, Nihon University School of Dentistry at Matsudo Department of Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo

• Mega Jun-ichi

  Department of Dentistry for the Disabled, Nihon University School of Dentistry at Matsudo Department of Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo

抄録

Since the periodontal ligament (PDL) contains a heterogeneous cell population, it is challenging to identify all cell types within the tissue and to determine whether they function alone to produce tissue components or interact with other cell types. Further, it is difficult to isolate and expand single cell clones from PDL cells, as normal cells have a limited life span and are phenotypically unstable. In the present study, we inserted the human telomerase reverse transcriptase (hTERT) gene, which encodes the catalytic subunit of the telomerase holoenzyme, into normal human periodontal ligament (HPL) cells and successfully obtained single cell clones. Expression of the inserted gene and telomerase activity in each of the clones was confirmed. Unlike the original HPL cells, at the end of the study (day 120), clone populations continued to actively double without phenotypic alteration. Osteogenic characteristics were present in some but not all clones. In conclusion, immortalization of HPL cells was successfully accomplished by transduction with the hTERT gene. This is the first report of immortalization of different cell types derived from PDL. (J. Oral Sci. 47, 177-184, 2005)

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ｏｒａｌ　Ｓｃｉｅｎｃｅ

  Ｊｏｕｒｎａｌ　ｏｆ　Ｏｒａｌ　Ｓｃｉｅｎｃｅ 47 (4), 177-184, 2005

  日本大学歯学部

キーワード

• periodontal ligament cell
• hTERT
• immortalization
• single cell clone

詳細情報

• CRID: 1390001205044980608
• NII論文ID: 130000072751
• DOI: 10.2334/josnusd.47.177
• ISSN: 18804926
• ISSN: 13434934
• Web Site: https://www.jstage.jst.go.jp/article/josnusd/47/4/47_4_177/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2006220077
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可