Neuronal Nitric Oxide Synthase Is Crucial for Ganglion Cell Death in Rat Retinal Explant Cultures

• Katsuki Hiroshi

  Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University

• Yamamoto Rie

  Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University

• Nakata Daisuke

  Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University

• Kume Toshiaki

  Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University

• Akaike Akinori

  Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University

Abstract

We examined possible involvement of nitric oxide synthase (NOS) on ganglion cell death in explant cultures of neonatal rat retina. Survival of retinal ganglion cells was significantly prolonged by a broad-spectrum NOS inhibitor N^ω-nitro-<sc>L</sc>-arginine methylester. NADPH diaphorase staining revealed a diffused distribution of NOS activity in neuropils of the inner plexiform layer as well as several neurons in the inner nuclear layer. Moreover, 7-nitroindazole but not aminoguanidine promoted the survival of retinal ganglion cells. These results suggest a crucial role of neuronal NOS-derived nitric oxide in retinal ganglion cell death.<br>

Journal

• Journal of Pharmacological Sciences

  Journal of Pharmacological Sciences 94 (1), 77-80, 2004

  The Japanese Pharmacological Society

Keywords

• nitric oxide
• organotypic culture
• retina

Details

• CRID: 1390282680153587968
• NII Article ID: 130000073887
• NII Book ID: AA11806667
• DOI: 10.1254/jphs.94.77
• ISSN: 13478648
• ISSN: 13478613
• ISSN: http://id.crossref.org/issn/00223549
• NDL BIB ID: 6819291
• PubMed: 14745122
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6819291
• Web Site: https://search.jamas.or.jp/link/ui/2004120221
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed