Enhancement of Sphingosine 1-Phosphate-Induced Phospholipase C Activation During G0-G1 Transition in Rat Hepatocytes
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- Im Dong-Soon
- Laboratory of Pharmacology, College of Pharmacy, Pusan National University
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- Tomura Hideaki
- Laboratory of Signal Transduction, Institute for Molecular and Cellular Regulation, Gunma University
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- Tobe Masayuki
- Laboratory of Signal Transduction, Institute for Molecular and Cellular Regulation, Gunma University
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- Sato Koichi
- Laboratory of Signal Transduction, Institute for Molecular and Cellular Regulation, Gunma University
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- Okajima Fumikazu
- Laboratory of Signal Transduction, Institute for Molecular and Cellular Regulation, Gunma University
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We previously reported that sphingosine 1-phosphate (S1P) induces inhibition of adenylyl cyclase and activation of phospholipase C via independent G protein-coupled receptors in adult rat hepatocytes. Although S1P activation of phospholipase C and subsequent increase of intracellular Ca2+ concentration were enhanced during the primary culture of hepatocytes, S1P inhibition of adenylyl cyclase remained unchanged. Here, we addressed whether enhancement of S1P-induced actions is dependent on change of status from the differentiated (G0) phase to proliferating (G1/S) phase in hepatocytes. By employing cell-density-dependency of the transition (G0-G1) of hepatocytes in primary culture in vitro, it was found that the enhancement of phospholipase C activation by S1P was dependent on cell density and correlated to the G0-G1 transition. The correlation was further confirmed in vivo by 70% hepatectomy as a proliferating hepatocytes model. Northern blot analysis suggested an enhanced expression of S1P2 receptor in proliferating hepatocytes.<br>
収録刊行物
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- Journal of Pharmacological Sciences
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Journal of Pharmacological Sciences 95 (2), 284-290, 2004
公益社団法人 日本薬理学会
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詳細情報 詳細情報について
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- CRID
- 1390001205177693824
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- NII論文ID
- 10013295125
- 130000074377
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- NII書誌ID
- AA11806667
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- ISSN
- 13478648
- 13478613
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- NDL書誌ID
- 6978180
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- PubMed
- 15215654
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 使用不可