Addition of Maitake D-fraction Reduces the Effective Dosage of Vancomycin for the Treatment of Listeria-Infected Mice.
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- Kodama Noriko
- Department of Microbial Chemistry, Kobe Pharmaceutical University
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- Yamada Maho
- Department of Microbial Chemistry, Kobe Pharmaceutical University
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- Nanba Hiroaki
- Department of Microbial Chemistry, Kobe Pharmaceutical University
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Maitake D-fraction, β1,6-glucan having β1,3-branches, has been reported to activate the immune system of the host. To elucidate whether the D-fraction can reduce the clinical effective dosage of antibiotics in the treatment of opportunistic bacterial infection, we examined the effects of D-fraction on the treatment of Listeria monocytogenes-infected mice in combination with vancomycine (VCM), the only antibiotic used for methicillin-resistant Staphylococcus aureus (MRSA). Listeria-infection was introduced by its inoculation into the abdominal cavity of mice. Without treatment, all inoculated mice died within 3 days after the inoculation. In contrast, in the mice treated with combined therapy of D-faction (10 mg/kg per day) and VCM (10 mg/kg per day), the survival rate was maintained at 60% on the 10th day after the inoculation, which was superior to that of mice treated with VCM alone (10 mg/kg per day). To investigate the mechanism underlying the reinforcement of VCM treatment by the D-fraction, the activities of macrophages and splenic T cells of Listeria-infected mice were evaluated. In mice administered with both D-fraction and VCM, macrophages produced 2.7 times as much interleukin-1 as that of non-treated control mice. The bactericidal activity of splenic T cells was also enhanced by 2.6 times of that of non-treated control mice. These results indicate that D-fraction activates immuno-competent cells, induced cytokine production, and consequently enhanced the bactericidal activities of the splenic T cells against Listeria monocytogenes, suggesting the clinical benefit of D-fraction in the case of anti-bacterial treatment for patients with high risks.
収録刊行物
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- Jpn.J.Pharmacol.
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Jpn.J.Pharmacol. 87 (4), 327-332, 2001
公益社団法人 日本薬理学会
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詳細情報 詳細情報について
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- CRID
- 1390001204286433152
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- NII論文ID
- 130000078305
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- NII書誌ID
- AA00691188
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- COI
- 1:CAS:528:DC%2BD3MXptlKku78%3D
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- ISSN
- 13473506
- 00215198
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- NDL書誌ID
- 6019318
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- PubMed
- 11829152
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可