Nitric Oxide Production and Smooth Muscle Cell Phenotype of Endothelial Cell-Smooth Muscle Cell Cocultured Model
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- SAKAMOTO Naoya
- Department of Bioengineering and Robotics, Graduate School of Engineering, Tohoku University
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- OI Masaki
- Department of Bioengineering and Robotics, Graduate School of Engineering, Tohoku University
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- OHASHI Toshiro
- Department of Bioengineering and Robotics, Graduate School of Engineering, Tohoku University
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- SATO Masaaki
- Department of Bioengineering and Robotics, Graduate School of Engineering, Tohoku University
Abstract
This study investigated nitric oxide (NO) production and phenotype changes of smooth muscle cells (SMC) in a cocultured model (CM) exposed to fluid shear stress. The CM was composed of human umbilical endothelial cells (EC) and SMC, a collagen layer, and a porous membrane. After exposing the CM to shear stress of 1.5 Pa for 24 hours, α-smooth muscle actin (α-SMA) expression of SMC and NO production in culture media were examined. Under static conditions, α-SMA expression in the CM was significantly lower than that of a SMC monocultured model (SMC model). After exposure to shear stress, NO production in the CM increased compared to that in the static CM, and there was no significant difference in α-SMA expression between the CM and SMC model. These results suggest that EC may regulate phenotype changes of cocultured SMC, and NO may be one of the factors which induce dedifferentiation of SMC.
Journal
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- Journal of Biomechanical Science and Engineering
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Journal of Biomechanical Science and Engineering 1 (1), 224-233, 2006
The Japan Society of Mechanical Engineers
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Keywords
Details 詳細情報について
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- CRID
- 1390282680238709376
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- NII Article ID
- 130000079545
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- ISSN
- 18809863
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed