Expression of the Heparin-Binding Growth Factor Midkine in the Cerebrospinal Fluid of Patients with Neurological Disorders

• Yoshida Yoshihiro

  School of Health Sciences, Faculty of Medicine, Kagoshima University

• Ikematsu Shinya

  Meiji Cell Technology Center, Meiji Milk Products Co. Ltd.

• Muramatsu Hisako

  Department of Biochemistry, School of Medicine, Nagoya University

• Sakakima Harutoshi

  School of Health Sciences, Faculty of Medicine, Kagoshima University

• Mizuma Nobuhisa

  Department of Neurology, Faculty of Medicine, Kagoshima University

• Matsuda Fumiyo

  School of Health Sciences, Faculty of Medicine, Kagoshima University

• Sonoda Ken

  Kagoshima Medical Association Hospital

• Umehara Fujio

  Department of Neurology, Faculty of Medicine, Kagoshima University

• Ohkubo Ryuichi

  Department of Neurology, Faculty of Medicine, Kagoshima University

• Matsuura Eiji

  Department of Neurology, Faculty of Medicine, Kagoshima University

• Goto Masamichi

  Department of Pathology, Faculty of Medicine, Kagoshima University

• Osame Mitsuhiro

  Department of Neurology, Faculty of Medicine, Kagoshima University

• Muramatsu Takashi

  Department of Biochemistry, School of Medicine, Nagoya University

Abstract

Objective This study was to clarify the roles of midkine (MK) in the brain.<br> Methods We determined cerebrospinal fluid MK levels in patients with neurological disorders by enzyme-linked immunoassay and immunostained autopsied brain samples in patients with meningitis.<br> Results MK levels were 0.37±0.21 ng/ml in controls (n=46, mean ± S.D.), 0.67±0.19 ng/ml in patients with cerebral infarction (n=8), 1.78±1.32 ng/ml in patients with meningitis (n=25; ANOVA and post-hoc Fisher's PLSD test, p<0.0001), 0.31±0.25 ng/ml in patients with human T-lymphotrophic virus type I-associated myelopathy/tropical spastic paraparesis (n=29), and 0.42±0.17 ng/ml in patients with amyotrophic lateral sclerosis (n=8). The regression equations were Y=0.005X+0.498 (Y, CSF MK level; X, cell number) and Y=0.007X+0.326 (Y, MK level; X, protein level) for all CSF samples. Autopsy brain samples from patients with meningitis expressed MK weakly in mononuclear cells on immunohistochemical examination. Western blot and polymerase chain reaction analyses showed that leukocytes were MK positive. CSF MK levels were not high in patients with cerebral infarction but were increased in patients with meningitis. CSF MK levels were high in normal controls, compared to those of other cytokines. MK was expressed in choroid plexus of normal brain and released there.<br> Conclusion Our findings suggested that MK may maintain normal adult brain as a neurotrophic factor, and that MK may be released from leucocytes in brain of patients with meningitis as an immunological mediator.<br>

Journal

• Internal Medicine

  Internal Medicine 47 (2), 83-89, 2008

  The Japanese Society of Internal Medicine

Keywords

• CSF
• MK
• neurotrophic factor
• cytokine
• meningitis
• cerebral infarction

Details

• CRID: 1390001204871594368
• NII Article ID: 130000079613
• DOI: 10.2169/internalmedicine.47.0260
• ISSN: 13497235
• ISSN: 09182918
• Web Site: http://www.jstage.jst.go.jp/article/internalmedicine/47/2/47_2_83/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2008229756
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed