Light scattering from assemblies of an archaeal feast/famine regulatory protein, DM1 (pot1216151)
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- SAKUMA Michiyo
- National Institute of Advanced Industrial Science and Technology (AIST) Japan Science and Technology Agency (JST), Cooperative System for Supporting Priority Research (CSSPR)
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- NAKAMURA Masahide
- Sugito Laboratory, Shoko Co.
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- KOIKE Hideaki
- National Institute of Advanced Industrial Science and Technology (AIST)
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- SUZUKI Masashi
- National Institute of Advanced Industrial Science and Technology (AIST)
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Abstract
Light scattered by assemblies of a feast/famine regulatory protein (FFRP), pot1216151 (DM1) from the hyperthermophilic archaeon Pyrococcus sp. OT3, was measured by combining an multi-angle detector with a gel filtration device. Four peaks were separated by gel filtration. For peak 4, the molecular weight (MW) was estimated as ~20K, a value not so different from that of dimers, 17K, by measuring static light scattering. For peak 3, MW was estimated as ~33K, suggesting formation of tetramers of MW of 34K. Peak 2 was the smallest among the four peaks, and not clearly separated from nearby peak 3, upon measuring light scattering. While, MW estimated for peak 1 was 112-118K, an intermediate between that of octamer, 69K, and that of hexadecamers, 138K. On the basis of these findings transition between assembly forms is discussed.<br> <br> <br> (Communicated by Masanori OTSUKA, M.J.A.)<br>
Journal
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- Proceedings of the Japan Academy, Series B
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Proceedings of the Japan Academy, Series B 81 (4), 110-116, 2005
The Japan Academy
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Details 詳細情報について
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- CRID
- 1390282679122978944
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- NII Article ID
- 130000093807
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- NII Book ID
- AA00785485
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- ISSN
- 13492896
- 03862208
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- NDL BIB ID
- 7465005
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed