A SELEX study of the DNA-binding specificity of archaeal FFRPs:2. FL4 (pot1613368)

• YOKOYAMA Katsushi

• IHARA Michiko

• EBIHARA Sonomi

• SUZUKI Masashi

  National Institute of Advanced Industrial Science and Technology (AIST)

書誌事項

タイトル別名

• SELEX study of the DNA binding specificity of archaeal FFRPs 2 FL4 pot1613368

抄録

The DNA-binding specificity of a transcription factor, the FFRP FL4 (pot1613368) from Pyrococcus sp. OT3, was studied. Using SELEX (systematic evolution of ligands by exponential environment) experiments, from a set of fragments, ∼150 bps, of the genomic DNA of P. OT3, seven were selected as containing binding sites. Thirteen bases identified as shared by the seven selected fragments with the least mismatches, 2.71 on average, was ATGAAAAAG TCAT. This sequence was closely related with another sequence, ATGAA[AAA/TTT]TTCAT, in the 5-3-5 arrangement, i.e. N_AN_BN_CN_DN_E[AAA/TTT]N_EN_DN_CN_BN_A, where, e.g. N_A was the base complementary to N_A. The average number of mismatches found between this sequence and the seven fragments was 3.14. A sequence, TTGAAATTTA CAA, resembling the sequence ATGAA[AAA/TTT] TTCAT and also another 5-3-5 sequence, TTGAA[AAA/ TTT]TTCAA, was found upstream of the fl4 gene, which is potentially recognized by FL4 for auto-regulation. Thus it is likely that an ideal binding-site of FL4 is ATGAA[AAA/TTT]TTCAT or TTGAA[AAA/TTT]TTCAA. In this abstract, the sequences were highlighted in Italic at 3, and with bold characters at 5 and 5. When two sequences compared were the same at some positions, there they were underlined.<br> <br> <br> (Communicated by Masanori OTSUKA, M.J.A.)<br>

収録刊行物

• Ｐｒｏｃｅｅｄｉｎｇｓ　ｏｆ　ｔｈｅ　Ｊａｐａｎ　Ａｃａｄｅｍｙ．　Ｓｅｒ．　Ｂ：　Ｐｈｙｓｉｃａｌ　ａｎｄ　Ｂｉｏｌｏｇｉｃａｌ　Ｓｃｉｅｎｃｅｓ

  Ｐｒｏｃｅｅｄｉｎｇｓ　ｏｆ　ｔｈｅ　Ｊａｐａｎ　Ａｃａｄｅｍｙ．　Ｓｅｒ．　Ｂ：　Ｐｈｙｓｉｃａｌ　ａｎｄ　Ｂｉｏｌｏｇｉｃａｌ　Ｓｃｉｅｎｃｅｓ 82 (1), 33-44, 2006

  日本学士院

キーワード

• Archaea
• AsnC
• DNA-protein interaction
• hyper-thermophile
• Lrp
• transcription factor.

詳細情報

• CRID: 1390282679123621376
• NII論文ID: 130000093866
• NII書誌ID: AA00785485
• DOI: 10.2183/pjab.82.33
• ISSN: 13492896
• ISSN: 03862208
• NDL書誌ID: 7985352
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7985352
• Web Site: http://www.jstage.jst.go.jp/article/pjab/82/1/82_1_33/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
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