Cysteine-390 is the binding site of luminous substance with symplectin, a photoprotein from Okinawan squid, Symplectoteuthis oualaniensis

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Author(s)

Abstract

Symplectin is a photoprotein from a luminous squid, <I>Symplectoteuthis oualaniensis</I>. It has a luminous substrate, dehydrocoelenterazine (DCZ), linked through a thioether bond with a cysteine residue. We have proven the binding site of luminous substrate in symplectin by using an artificial analogue of DCZ, <I>ortho</I>-fluoro-DCZ (F-DCZ). F-DCZ-symplectin emitting strong blue light was reconstituted from apo-symplectin and F-DCZ. Proteolytic digestion of the reconstituted F-DCZ-symplectin afforded peptides including C<SUP>390</SUP>GLK-F-DCZ (amide), which was detected with a house assembled nano-LC-ESI-Q-TOF-MS. The chromo-peptide derived from the F-DCZ-symplectin after luminescence showed the lower molecular mass than that before the luminescence by 12 mass units, corresponding to the loss of one carbon atom upon emitting light. Thus, we have concluded that F-DCZ analogue binds to Cys390 in symplectin so as to emit light.<BR><BR>(Edited by Teruhiko BEPPU, M.J.A.)

Journal

  • Proceedings of the Japan Academy, Series B

    Proceedings of the Japan Academy, Series B 84(9), 386-392, 2008

    The Japan Academy

Codes

  • NII Article ID (NAID)
    130000093921
  • NII NACSIS-CAT ID (NCID)
    AA00785485
  • Text Lang
    ENG
  • ISSN
    0386-2208
  • NDL Article ID
    9750539
  • NDL Source Classification
    ZS8(科学技術--医学--解剖学・生理学・生化学) // ZR1(科学技術--生物学)
  • NDL Call No.
    Z53-T495
  • Data Source
    NDL  J-STAGE 
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