Mechanism of insulin uptake in rat alveolar type 2 and type 1-like epithelial cells

• Ikehata Mika

  Department of Pharmaceutics and Therapeutics, Graduate School of Biomedical Sciences, Hiroshima University

• Yumoto Ryoko

  Department of Pharmaceutics and Therapeutics, Graduate School of Biomedical Sciences, Hiroshima University

• Kato Yuki

  Department of Pharmaceutics and Therapeutics, Graduate School of Biomedical Sciences, Hiroshima University

• Nagai Junya

  Department of Pharmaceutics and Therapeutics, Graduate School of Biomedical Sciences, Hiroshima University

• Takano Mikihisa

  Department of Pharmaceutics and Therapeutics, Graduate School of Biomedical Sciences, Hiroshima University

書誌事項

タイトル別名

• Mechanism of Insulin Uptake in Rat Alveolar Type II and Type I-Like Epithelial Cells
• Mechanism of insulin uptake in rat alveolar type II and type I-like epitherial cells

抄録

The mechanism underlying the handling of protein and peptide drugs such as insulin in alveolar epithelial cells is still unclear. We therefore examined fluorescein isothiocyanate-labeled (FITC)-insulin uptake in rat primary cultured alveolar type II epithelial cells and in transdifferentiated type I-like cells. FITC-insulin uptake in these cells was much higher than those of FITC-immunoglobulin (IgG), transferrin, and dextran. FITC-insulin uptake was time- and concentration-dependent, and was almost completely inhibited by metabolic inhibitors in both cells, while bafilomycin A₁ inhibited the uptake only in type II cells. Inhibitors of clathrin- and caveolae-mediated endocytosis did not affect FITC-insulin uptake in either type of cell. Dynasore, a dynamin GTPase inhibitor, potently inhibited FITC-insulin uptake in type II cells. These results suggest that the characteristics of insulin uptake in type II and type I cells are different, and dynamin-dependent endocytosis that utilizes neither clathrin nor caveolae is involved in type II cells, while a dynamin-independent pathway is mainly involved in type I cells.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 32 (10), 1765-1769, 2009

  公益社団法人 日本薬学会

キーワード

• insulin
• alveolar epithelial cell
• endocytosis
• primary culture
• dynamin

詳細情報

• CRID: 1390001204624918016
• NII論文ID: 130000116940
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.32.1765
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 10375942
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10375942
• Web Site: http://www.jstage.jst.go.jp/article/bpb/32/10/32_10_1765/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2010089388
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
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• 抄録ライセンスフラグ: 使用不可