Isolation of Sulfated Galactan from Codium fragile and Its Antiviral Effect
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- Ohta Yuko
- Graduate School of Medicine and Pharmaceutical Sciences for Research, University of Toyama
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- Lee Jung-Bum
- Graduate School of Medicine and Pharmaceutical Sciences for Research, University of Toyama
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- Hayashi Kyoko
- Graduate School of Medicine and Pharmaceutical Sciences for Research, University of Toyama
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- Hayashi Toshimitsu
- Graduate School of Medicine and Pharmaceutical Sciences for Research, University of Toyama
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Abstract
A sulfated galactan (FG) was isolated from the green alga, Codium fragile. Chemical analysis revealed that FG mainly consisted of D-galactose with pyruvic acid (12.3%) and sulfate (11.0%). Methylation and NMR analyses showed that FG was composed of →3)-β-D-Galp-(1→, β-D-Galp-(1→ and →3,6)-β-D-Galp-(1→ residue. In addition, pyruvic acid was suggested to be present as (1′-carboxy)-ethylidene cyclic ketal at O-3 and O-4 of non-reducing terminal galactose residues, whereas sulfate was substituted at O-4 of other galactose residues. When the antiviral potency of FG was evaluated, it inhibited the replication of herpes simplex virus type 2 (HSV-2), and the mechanism of action was suggested to be interference in the early steps such as virus adsorption to and penetration into host cells. Furthermore, it was shown that FG directly reduced virus infection rates. In a genital infection model using HSV-2-infected mice, FG improved mortality and lesion scores and reduced virus yields by intravaginal administration. These results suggest that FG might be a potent candidate as a prophylactic agent for HSV-2 infection.
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 32 (5), 892-898, 2009
The Pharmaceutical Society of Japan
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Details 詳細情報について
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- CRID
- 1390001204627467008
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- NII Article ID
- 130000117156
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- NII Book ID
- AA10885497
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- COI
- 1:CAS:528:DC%2BD1MXmsVGlsb4%3D
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- ISSN
- 13475215
- 09186158
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- NDL BIB ID
- 10212601
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- PubMed
- 19420760
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed