Phospho-Nbs1 and Mre11 proteins which recognize DSBs co-localize with γH2AX in the nucleus after heat treatment
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- Takahashi Akihisa
- Department of Biology, Nara Medical University School of Medicine
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- Mori Eiichiro
- Department of Biology, Nara Medical University School of Medicine
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- Ohnishi Takeo
- Department of Biology, Nara Medical University School of Medicine
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Abstract
Background: Critical hyperthermia targets in cancer cells have to be clearly identified.<br>Method: To determine if the DNA double strand break (DSB)-recognizing proteins, phospho-Nbs1 and Mre11, co-localize with phospho-H2AX (γH2AX), immunohistochemical methods were used with multiple antibodies. After a heat treatment at 45.5°C for 20 min, human normal fibroblasts were analyzed with a laser scanning confocal microscope at 0.5 h and 8 h post-heat treatment. <br>Results: At 0.5 h after a heat treatment, γH2AX formed foci in the nucleus, but phospho-Nbs1 and Mre11 were scattered over the nucleus and cytoplasm, and did not co-localize with γH2AX. At 8 h after a heat-treatment, and also after X-irradiation, both phospho-Nbs1 and Mre11 co-localized with γH2AX foci, which were already present, in the nucleus. Moreover, both phospho-Nbs1 and Mre11 which were observed at 0.5 h after a heat treatment in the cytoplasm, were no longer observed in the cytoplasm at 8 h after a heat treatment.<br>Conclusion: These findings provide support for the concept that heat, like X-rays, may lead to the induction of DSBs.
Journal
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- Annals of Cancer Research and Therapy
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Annals of Cancer Research and Therapy 15 (2), 50-53, 2007
The Japanese Society of Strategies for Cancer Research and Therapy
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Details 詳細情報について
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- CRID
- 1390001205190687104
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- NII Article ID
- 130000119681
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- ISSN
- 18805469
- 13446835
- http://id.crossref.org/issn/13446835
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed
