Purification and Identification of Angiotensin I-Converting Enzyme Inhibitor from Morokheiya (Corchorus olitorius).

• KIMOTO Koichi

  Nutritional Biochemistry, Tokyo Kasei University

• KURODA Yuko

  Nutritional Biochemistry, Tokyo Kasei University

• SAITO Yumiko

  Nutritional Biochemistry, Tokyo Kasei University

• YAMAMOTO Junko

  Nutritional Biochemistry, Tokyo Kasei University

• MURAKAMI Tetsuo

  Food and Nutritional Chemistry, Kinki University

• AOYAGI Yasuo

  Food Chemistry, Women's College of Nutrition

抄録

In order to invesigate another function such as food in Morokheiya, the angiotensin-converting enzyme (ACE) inhibitor was extracted with 80% ethanol from the leaves of Morokheiya (Corchorus olitorious). The extract was dried, dissolved in water, defatted with ether and decolored with activated carbon. The ACE inhibitor was purified by successive ion-exchange chromatography, Amberlite IR-120B, Dowex 50W-X8 and Dowex 1-X4, respectively. The ACE inhibitor was further purified by silica gel column chromatography and finally purified and isolated by high performance liquid chromatography (HPLC) on Asahipak NH2P-50. The ACE inhibitor showed a positive ninhydrin reaction and no significant absorbance. The present ACE inhibitor was identified as Nicotianamine based on the comparative study using amino acid analyzer, TLC and capillary electrophoresis.

収録刊行物

• Food Science and Technology International, Tokyo

  Food Science and Technology International, Tokyo 4 (3), 223-226, 1998

  社団法人 日本食品科学工学会

キーワード

• ACE inhibitor
• amino acid
• nikotianamine

詳細情報

• CRID: 1390001204456748672
• NII論文ID: 130000122914
• DOI: 10.3136/fsti9596t9798.4.223
• COI: 1:CAS:528:DyaK1cXnsFeksr0%3D
• ISSN: 18813976
• ISSN: 13417592
• ISSN: http://id.crossref.org/issn/13417592
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可