Osteopontin Modulates Malignant Pleural Mesothelioma Cell Functions in Vitro

• Ohashi Rina

  Department of Respiratory Medicine, Juntendo University, School of Medicine Research Institute for Disease of Old Ages, Juntendo University, School of Medicine

• Tajima Ken

  Department of Respiratory Medicine, Juntendo University, School of Medicine Research Institute for Disease of Old Ages, Juntendo University, School of Medicine

• Cui Ri

  Department of Respiratory Medicine, Juntendo University, School of Medicine Research Institute for Disease of Old Ages, Juntendo University, School of Medicine

• Gu Tao

  Department of Respiratory Medicine, Juntendo University, School of Medicine Research Institute for Disease of Old Ages, Juntendo University, School of Medicine

• Hino Okio

  Department of Pathology and Oncology, Juntendo University, School of Medicine

• Shiomi Kazu

  Department of General Thoracic Surgery, Juntendo University, School of Medicine

• Miyamoto Hideaki

  Department of General Thoracic Surgery, Minami-Tohoku Hospital

• Nishio Kazuto

  Department of Genome Biology, Kinki University School of Medicine

• Takahashi Kazuhisa

  Department of Respiratory Medicine, Juntendo University, School of Medicine Research Institute for Disease of Old Ages, Juntendo University, School of Medicine

Bibliographic Information

Other Title

• オステオポンチンは悪性胸膜中皮腫の細胞機能に影響を与える

Abstract

Objective. It has been reported that serum osteopontin (OPN) levels of persons with exposure to asbestos who have malignant pleural mesothelioma (MPM) are increasing and useful for early diagnosis of MPM. OPN contains binding sites for several receptors including αvβ3 integrins, which are thought to play various roles in mediating cell-matrix interactions. The aim of this study is to evaluate roles of OPN in MPM cell line. Methods. With MPM cell lines, we conducted reverse transcriptase-polymerase chain reaction (RT-PCR) to evaluate OPN mRNA expression. Expression of integrins on the surface of this cell line were analyzed with a FACScan^TM. To evaluate cell adhesion and proliferation mediated by OPN, cells were added to 96-well flat bottom plates coated with OPN, bovine serum albumin (BSA), poly-L-lysine (PLL) or hyaluronate (HA). Adherent and viable cells were counted with cell counting kit-8^TM. To evaluate phosphorylation of focal adhesion kinase (FAK) in H28 cells on OPN, we performed immunoprecipitation and immunoblotting. Apoptotic cells cultured on these plates were detected by the binding of annexin V. Results. OPN and αvβ3 expression was detected with both RT-PCR and FACScan^TM, respectively. H28 cells adhered to OPN, PLL, or HA to much greater extent than to BSA. However, H28 cells cultured on OPN coated plates showed enhanced proliferation, but not on BSA, PLL or HA. In addition, high level of phosphorylated FAK in H28 cells plated on OPN was observed. Furthermore, less apoptotic cells were revealed cultured on OPN coated plates in comparison to others. Conclusion. In conclusion, OPN may play an important role in the enhancement of adhesion and proliferation of H28 cells, presumably by interacting with αvβ3 integrins.<br>

Journal

• Haigan

  Haigan 49 (4), 368-375, 2009

  The Japan Lung Cancer Society

Keywords

• Malignant pleural mesothelioma
• Osteopontin
• αvβ3 integrin

Details

• CRID: 1390001204682129024
• NII Article ID: 130000122999
• DOI: 10.2482/haigan.49.368
• ISSN: 13489992
• ISSN: 03869628
• Web Site: http://www.jstage.jst.go.jp/article/haigan/49/4/49_4_368/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2010014184
• Text Lang: ja
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed