Molecular Cloning of Canine Protease-Activated Receptor-2 and its Expression in Normal Dog Tissues and Atopic Skin Lesions

• MAEDA Shingo

  Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University

• MAEDA Sadatoshi

  Department of Veterinary Internal Medicine, Faculty of Applied Biological Sciences, Gifu University

• SHIBATA Sanae

  The United Graduate School of Veterinary Sciences, Gifu University

• CHIMURA Naoki

  Department of Veterinary Internal Medicine, Faculty of Applied Biological Sciences, Gifu University

• FUKATA Tsuneo

  The United Graduate School of Veterinary Sciences, Gifu University

Bibliographic Information

Other Title

• Internal medicine: Molecular cloning of canine protease-activated receptor-2 and its expression in normal dog tissues and atopic skin lesions

Abstract

Protease-activated receptor-2 (PAR-2) belongs to a new G protein-coupled receptor subfamily and is activated by serine proteases. PAR-2 has been demonstrated to play an important role in inflammation and immune response in allergic diseases. In this study, we cloned canine PAR-2 cDNA from the canine kidney by RT-PCR. The canine PAR-2 clone contained a full-length open reading frame encoding 397 amino acids that had 84% and 80% homology with human and mouse homologues, respectively. Canine PAR-2 mRNA was detected in the heart, lung, liver, pancreas, stomach, small intestine, colon, kidney, adrenal gland, spleen, thyroid gland, thymus, skeletal muscle, lymph node, fat and skin of three healthy dogs. The expression pattern of PAR-2 mRNA in canine tissues was similar to that in humans. The expression level of PAR-2 mRNA in skin was not different between the atopic dermatitis (AD) and healthy dogs, suggesting that the level of PAR-2 mRNA transcription may not be associated with development of canine AD. The canine PAR-2 cDNA clone obtained in this study will be useful for further investigation of the immunopathogenesis of canine allergic diseases.<br>

Journal

• Journal of Veterinary Medical Science

  Journal of Veterinary Medical Science 71 (5), 577-582, 2009

  JAPANESE SOCIETY OF VETERINARY SCIENCE

Keywords

• atopic dermatitis
• canine
• molecular cloning
• protease-activated receptor-2

Details

• CRID: 1390001206430814976
• NII Article ID: 130000134367
• NII Book ID: AA10796138
• DOI: 10.1292/jvms.71.577
• ISSN: 13477439
• ISSN: 09167250
• HANDLE: 20.500.12099/39496
• NDL BIB ID: 10314305
• Web Site: https://gifu-u.repo.nii.ac.jp/records/78770
• Web Site: http://id.ndl.go.jp/bib/10314305
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10314305
• Web Site: http://www.jstage.jst.go.jp/article/jvms/71/5/71_5_577/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2009241153
• Text Lang: en
• Data Source:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed