Determination of Full-Length cDNA Nucleotide Sequence of Equine Carbonic Anhydrase VI and Its Expression in Various Tissues
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- OCHIAI Hideharu
- Research Institute of Biosciences, Azabu University
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- KANEMAKI Nobuyuki
- Veterinary Teaching Hospital, Azabu University, School of Veterinary Medicine
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- KAMOSHIDA Shino
- Laboratory of Molecular Biology, Azabu University, School of Veterinary Medicine
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- MURAKAMI Masaru
- Laboratory of Molecular Biology, Azabu University, School of Veterinary Medicine
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- ICHIHARA Nobutsune
- Laboratory of Veterinary Anatomy 1, Azabu University, School of Veterinary Medicine
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- ASARI Masao
- Laboratory of Veterinary Anatomy 1, Azabu University, School of Veterinary Medicine
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- NISHITA Toshiho
- Laboratory of Veterinary Physiology 1, Azabu University, School of Veterinary Medicine
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抄録
A full-length cDNA clone of an equine carbonic anhydrase (CA)-VI was obtained from the equine parotid gland. The cDNA sequence was 1338 bp long and was predicted to encode a 319 amino acid polypeptide with a putative signal peptide of 18 amino acids. The deduced amino acid sequence of mature CA-VI showed the similarity of 70% to those of other mammalians reported. Westernblot analysis using anti-horse CA-VI peptide detected the single band in parotid gland, and the band reduced its size by treatment with N-glycosidase F. Additionally, CA-VI protein expression was confirmed in submandicular gland and weakly in liver. In contrast, RT-PCR analysis revealed signals in the digestive tract including duodenum, jejunum, ileum, cecum and colon as well as the salivary glands. In addition, certain signals were detected in testis, thyroid gland and liver, but not in nerve tissue, skeletal muscle, spleen or lymph node.<br>
収録刊行物
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- Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｖｅｔｅｒｉｎａｒｙ Ｍｅｄｉｃａｌ Ｓｃｉｅｎｃｅ
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Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｖｅｔｅｒｉｎａｒｙ Ｍｅｄｉｃａｌ Ｓｃｉｅｎｃｅ 71 (9), 1233-1237, 2009
公益社団法人 日本獣医学会