Laboratory animal science: PEGylated lactoferrin enhances its hepatoprotective effects on acute liver injury induced by D-galactosamine and lipopolysaccharide in rats

• SUGIYAMA Akihiko

  Course of Veterinary Laboratory Medicine, School of Veterinary Medicine, Faculty of Agriculture, Tottori University

• SATO Atsushi

  School of Bioscience and Biotechnology, Tokyo University of Technology

• SHIMIZU Hirohiko

  NRL Pharma Inc.

• ANDO Kunio

  NRL Pharma Inc.

• TAKEUCHI Takashi

  Course of Veterinary Laboratory Medicine, School of Veterinary Medicine, Faculty of Agriculture, Tottori University

書誌事項

タイトル別名

• PEGylated Lactoferrin Enhances Its Hepatoprotective Effects on Acute Liver Injury Induced by D-Galactosamine and Lipopolysaccharide in Rats

抄録

Polyethylene glycol (PEG) is attached to proteins in order to increase their half-life in circulation and reduce their immunogenicity in vivo. The present study was conducted to examine whether two different sizes of PEGylated bovine lactoferrin (40k- and 20k-PEG-bLf) would enhance the protective effect of native bLf on liver injury induced by D-galactosamine (GalN) and lipopolysaccharide (LPS) in rats. The treatment of PEGylated bLf more remarkably prevented the elevation of serum levels of hepatic enzyme markers and inhibited inflammatory and hemorrhagic changes and hepatic apoptosis induced by GalN/LPS than native bLf. The treatment of PEGylated bLf more significantly inhibited the increased concentration of proinflammatory cytokines (TNF-α and IL-6) in serum caused by GaIN/LPS, and enhanced anti-inflammatory cytokine (IL-10) production more than native bLf. PEGylated bLf decreased serum levels of nitric oxide (NO) more than native bLf. These results indicate that PEGylated bLf inhibits more significantly the induction of inflammatory mediators such as cytokines and NO than native bLf, resulting in the enhancement of its prevention of fulminant liver failure induced by GalN/LPS in rats. The present study provided evidence that PEGylated bLf may offer a novel alternative therapy for the prevention of acute hepatic failure through its anti-inflammatory and immunomodulatory properties.<br>

収録刊行物

• Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ

  Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ 72 (2), 173-180, 2010

  公益社団法人 日本獣医学会

キーワード

• cytokines
• hepatoprotection
• lactoferrin
• PEGylated

詳細情報

• CRID: 1390001206428049920
• NII論文ID: 130000134526
• NII書誌ID: AA10796138
• DOI: 10.1292/jvms.09-0324
• ISSN: 13477439
• ISSN: 09167250
• NDL書誌ID: 10593003
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10593003
• Web Site: http://www.jstage.jst.go.jp/article/jvms/72/2/72_09-0324/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2010209749
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可