Inhibitory Effects of Flavonoid Glycosides Isolated from the Peel of Japanese Persimmon (Diospyros kaki 'Fuyu') on Melanin Biosynthesis

• Ohguchi Kenji

  Gifu International Institute of Biotechnology

• Nakajima Chizuru

  Gifu Pharmaceutical University

• Oyama Masayoshi

  Gifu Pharmaceutical University

• Iinuma Munekazu

  Gifu Pharmaceutical University

• Itoh Tomohiro

  Gifu International Institute of Biotechnology

• Akao Yukihiro

  Gifu International Institute of Biotechnology

• Nozawa Yoshinori

  Gifu International Institute of Biotechnology

• Ito Masafumi

  Gifu International Institute of Biotechnology

Abstract

We found that the acetone extract of the peel of Japanese persimmon (Diospyros kaki ‘Fuyu’) inhibits melanin biosynthesis in mouse B16 melanoma cells. The activity-guided purification of the extract resulted in isolation of two active compounds, which have been identified as flavonoid glycosides, isoquercitrin (quercetin-3-O-glucoside) and hyperin (quercetin-3-O-galactoside) by spectral analysis. Isoquercitrin and hyperin strongly inhibited the production of melanin (IC₅₀: 21.7 and 18.2 μM, respectively). The inhibitory effects were found to be mediated by suppression of tyrosinase expression.

Journal

• Biological and Pharmaceutical Bulletin

  Biological and Pharmaceutical Bulletin 33 (1), 122-124, 2010

  The Pharmaceutical Society of Japan

Keywords

• melanin
• tyrosinase
• persimmon
• kaki
• flavonoid

Details

• CRID: 1390282679601515264
• NII Article ID: 130000140204
• NII Article ID: 40016900226
• NII Book ID: AA10885497
• DOI: 10.1248/bpb.33.122
• ISSN: 13475215
• ISSN: 09186158
• NDL BIB ID: 10497538
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10497538
• Web Site: http://www.jstage.jst.go.jp/article/bpb/33/1/33_1_122/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2010153088
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed