Immunohistochemical Detection of 13(R)-hydroxyoctadecadienoic Acid in Atherosclerotic Plaques of Human Carotid Arteries Using a Novel Specific Antibody

• Shibata Noriyuki

  Department of Pathology, Tokyo Women’s Medical University

• Toi Sono

  Department of Neurology, Tokyo Women’s Medical University

• Shibata Takahiro

  Laboratory of Food and Biodynamics, Nagoya University Graduate School of Bioagricultural Sciences

• Uchida Koji

  Laboratory of Food and Biodynamics, Nagoya University Graduate School of Bioagricultural Sciences

• Itabe Hiroyuki

  Department of Biological Chemistry, Showa University School of Pharmaceutical Sciences

• Sawada Tatsuo

  Department of Pathology, Tokyo Women’s Medical University

• Kawamata Takakazu

  Department of Neurosurgery, Yachiyo Medical Center

• Okada Yoshikazu

  Department of Neurosurgery, Tokyo Women’s Medical University

• Uchiyama Shinichiro

  Department of Neurology, Tokyo Women’s Medical University

• Kobayashi Makio

  Department of Pathology, Tokyo Women’s Medical University

Abstract

13-Hydroxyoctadecadienoic acid (13-HODE) is a major component of oxidized low density lipoprotein (OxLDL), which has been shown to have a crucial role in atherogenesis. Of the 13-HODE stereoisomers, 13(S)-HODE and 13(R)-HODE, little is known about the latter in contrast to the former. To detect 13(R)-HODE in atherosclerotic lesions, we prepared a mouse monoclonal antibody against 13(R)-HODE. Competitive enzyme-linked immunosorbent assay clarified the selective reaction of a clone mAb 13H1 with both free and bovine serum albumin-conjugated forms of 13(R)-HODE but not other oxidized lipids including 13(S)-HODE. Immunohistochemical analysis revealed the colocalization of 13(R)-HODE immunoreactivity with the OxLDL marker oxidized phophatidylcholine immunoreactivity in vascular endothelial cells, macrophages and migrating vascular smooth muscle cells in atherosclerotic plaques of human carotid arteries. The present results provide in vivo evidence for the formation of 13(R)-HODE in atherosclerotic lesions of carotid arteries.<br>

Journal

• ACTA HISTOCHEMICA ET CYTOCHEMICA

  ACTA HISTOCHEMICA ET CYTOCHEMICA 42 (6), 197-203, 2009

  JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

Keywords

• carotid atherosclerosis
• enzyme-linked immunosorbent assay
• 13(R)-hydroxyoctadecadienoic acid
• immunohistochemistry
• monoclonal antibody

Details

• CRID: 1390001204863254912
• NII Article ID: 130000142919
• DOI: 10.1267/ahc.09022
• ISSN: 13475800
• ISSN: 00445991
• Web Site: http://www.jstage.jst.go.jp/article/ahc/42/6/42_09022/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2010309943
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed