Development of Reverse Transcription-Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for Detection of Avian Influenza Viruses in Field Specimens

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Author(s)

    • SHIVAKOTI Sakar
    • Laboratory of Veterinary Public Health, Faculty of Agriculture, Tottori University
    • ITO Hiroshi
    • Laboratory of Veterinary Public Health, Faculty of Agriculture, Tottori University|Avian Zoonosis Research Center, Faculty of Agriculture, Tottori University
    • MURASE Toshiyuki
    • Avian Zoonosis Research Center, Faculty of Agriculture, Tottori University|Laboratory of Veterinary Microbiology, Faculty of Agriculture, Tottori University
    • ONO Etsuro
    • Avian Zoonosis Research Center, Faculty of Agriculture, Tottori University|Laboratory of Biomedicine, Center of Biomedical Research, Graduate School of Medical Sciences, Kyushu University
    • YAMASHIRO Tetsu
    • Department of Virology, Institute of Tropical Medicine, Nagasaki University
    • OTSUKI Koichi
    • Avian Zoonosis Research Center, Faculty of Agriculture, Tottori University|Avian Influenza Research Center, Kyoto Sangyo University
    • ITO Toshihiro
    • Laboratory of Veterinary Public Health, Faculty of Agriculture, Tottori University|Avian Zoonosis Research Center, Faculty of Agriculture, Tottori University

Abstract

Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is an established gene amplification method for rapid diagnosis of various infectious diseases. In order to detect avian influenza viruses, particularly in field specimens, specific primers targeting the matrix gene were designed. Thirty-four virus samples, including isolates from wild and domestic avian hosts belonging to various geographical areas, were used to confirm the validity of the primers. All samples were confirmed to be positive in less than 1 hr. The RT-LAMP assay was also able to detect avian influenza virus in the various field samples, such as swabs, tissues, and feces. These results indicate that the developed RT-LAMP assay with uniquely designed primers is potentially useful in comprehensive avian influenza surveillance.<br>

Journal

  • Journal of Veterinary Medical Science

    Journal of Veterinary Medical Science 72(4), 519-523, 2010

    JAPANESE SOCIETY OF VETERINARY SCIENCE

Codes

  • NII Article ID (NAID)
    130000149956
  • Text Lang
    ENG
  • ISSN
    0916-7250
  • Data Source
    J-STAGE 
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