Cytochrome P450 3As Gene Expression and Testosterone 6.BETA.-Hydroxylase Activity in Human Fetal Membranes and Placenta at Full Term
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- Maezawa Kayoko
- Department of Pharmacy, Shinshu University Hospital
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- Matsunaga Tamihide
- Department of Pharmacy, Shinshu University Hospital
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- Takezawa Takashi
- Department of Pharmacy, Shinshu University Hospital
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- Kanai Makoto
- Department of Family and Child Nursing, and Midwifery
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- Ohira Satoshi
- Department of Obstetrics and Gynecology, Shinshu University School of Medicine
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- Ohmori Shigeru
- Department of Pharmacy, Shinshu University Hospital
Bibliographic Information
- Other Title
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- Cytochrome P450 3As gene expression and testosterone 6β-hydroxylase activity in human fetal membranes and placenta at full term
- Cytochrome P450 3As gene expression and testosterone 6 v hydroxylase activity in human fetal membranes and placenta at full term
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Abstract
Expression levels of cytochrome P450 (CYP) 3A4, CYP3A5 and CYP3A7 mRNAs in placentas and fetal membranes, which were split into amnion and chorion leave attached decidua (chorion/decidua), obtained from pregnant women with normal delivery (5 subjects) and Caesarean section (15 subjects) were determined. These CYP3A mRNAs were also expressed in amnion and chorion/decidua together with placenta, although the expression level of these mRNAs was strikingly different between subjects. The expression level of the CYP3A4 mRNA in the placenta was about 2-fold higher than those in amnion and chorion/decidua. On the other hand, the expression levels of CYP3A5 and CYP3A7 mRNAs were highest in chorion/decidua. The immunologically related protein(s) with CYP3A7 was detected in all tissues examined. Testosterone 6β-hydroxylase activity in homogenate of human placenta, amnion and chorion/decidua were 26.6, 3.7 and 4.6 pmol/h/mg protein, respectively. These results suggest that CYP3As in fetal membranes have the metabolic function to protect the fetus from exposure to drugs.
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 33 (2), 249-254, 2010
The Pharmaceutical Society of Japan
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Details 詳細情報について
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- CRID
- 1390001204625543424
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- NII Article ID
- 130000156632
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- NII Book ID
- AA10885497
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- COI
- 1:STN:280:DC%2BC3c%2FmvFejug%3D%3D
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- ISSN
- 13475215
- 09186158
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- HANDLE
- 10091/16950
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- NDL BIB ID
- 10536927
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- PubMed
- 20118548
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed