Renal secretion of urate by organic anion transporter 2 (OAT2/ SLC22A7) in human

• Sato Masanobu

  Kanazawa University, Institute of Medical, Pharmaceutical and Health Sciences, Faculty of Pharmacy

• Mamada Hideaki

  Kanazawa University, Institute of Medical, Pharmaceutical and Health Sciences, Faculty of Pharmacy

• Anzai Naohiko

  Department of Pharmacology and Toxicology, Kyorin University School of Medicine

• Shirasaka Yoshiyuki

  Kanazawa University, Institute of Medical, Pharmaceutical and Health Sciences, Faculty of Pharmacy

• Nakanishi Takeo

  Kanazawa University, Institute of Medical, Pharmaceutical and Health Sciences, Faculty of Pharmacy

• Tamai Ikumi

  Kanazawa University, Institute of Medical, Pharmaceutical and Health Sciences, Faculty of Pharmacy

書誌事項

タイトル別名

• Renal Secretion of Uric Acid by Organic Anion Transporter 2 (OAT2/SLC22A7) in Human
• Renal secretion of uric acid by organic anion transporter 2 (OAT2/SLO22A7) in human.

抄録

The physiological function of organic anion transporter OAT2 (SLC22A7) remains unclear, but since OAT2 transports purine derivatives, it may be involved in renal handling of uric acid, the final metabolite of purine derivatives. In the present study, we studied uric acid transport in stably OAT2-expressing HEK293 cells (HEK293/OAT2). OAT2 mediated uptake, but not efflux, of [¹⁴C]uric acid. Uric acid transport was saturable with K_m of 1168±335 μM (mean and S.E.M.) and V_max of 2.57±0.350 nmol/min/mg protein. The [¹⁴C]uric acid uptake was sensitive to Cl⁻ and was enhanced at acidic pH. In cis-inhibition assay, [¹⁴C]uric acid uptake was inhibited by several mono- or dicarboxylic acids, but it was not trans-stimulated by any of the compounds tested. The pattern of inhibition of OAT2-mediated uric acid transport by various drugs was different from that of OAT1- or OAT3-mediated transport. Furthermore, OAT2-mediated transport of uric acid was inhibited by an antiuricosuric drug, pyrazinecarboxylic acid. These results revealed distinct characteristics of uric acid transport via OAT2 compared with other uric acid transporters, suggesting that OAT2 plays a role in renal uric acid uptake from blood as a first step of tubular secretion. OAT2 may therefore be a potential target for regulating serum uric acid level.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 33 (3), 498-503, 2010

  公益社団法人 日本薬学会

キーワード

• organic anion transporter 2
• uric acid
• transporter
• uricosuric
• antiuricosuric
• serum uric acid level

詳細情報

• CRID: 1390001204625940608
• NII論文ID: 130000248105
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.33.498
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 10582662
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10582662
• Web Site: http://www.jstage.jst.go.jp/article/bpb/33/3/33_3_498/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2010238212
• 本文言語コード: en
• データソース種別:
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