Culturing Neurons on MEMS Fabricated P(VDF-TrFE) Films for Implantable Artificial Cochlea

Access this Article

Author(s)

    • SHINTAKU Hirofumi
    • Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University
    • TATENO Takashi
    • Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University
    • TSUCHIOKA Nobuyoshi
    • Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University
    • TANUJAYA Harto
    • Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University
    • NAKAGAWA Takayuki
    • Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University
    • ITO Juichi
    • Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University
    • KAWANO Satoyuki
    • Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University

Abstract

In this paper, we report an <I>in vitro</I> study on the biocompatibility of poly(vinylidene fluoride-trifluoroethylene) (P(VDF-TrFE)) films for the implantable artificial cochlea. The implantable artificial cochlea comprises a piezoelectric membrane made of P(VDF-TrFE), platinum (Pt) thin film electrodes, and a silicon substrate which are designed to stimulate neurons in a cochlea and fabricated by microelectromechanical systems (MEMS) and thin film technologies. The biocompatibility of P(VDF-TrFE) film is evaluated by culturing cerebral cortical neurons from rats on it. The fibronectin from human plasma and the collagen from the calf skin are used as the cell adhesion factors. Since neurons extend dendrites and axons from the somata, it is found that the neurons are successfully cultured on the surface of P(VDF-TrFE) films modified both by the fibronectin and by the collagen. Furthermore, it is also found that the neurons are also successfully cultured over the Pt electrode on the P(VDF-TrFE) of the implantable artificial cochlea modified by the fibronectin. Consequently, the biocompatibility and the applicability of the MEMS fabricated P(VDF-TrFE) films and the implantable artificial cochlea are confirmed.

Journal

  • Journal of Biomechanical Science and Engineering

    Journal of Biomechanical Science and Engineering 5(3), 229-235, 2010

    The Japan Society of Mechanical Engineers

Codes

  • NII Article ID (NAID)
    130000252220
  • Text Lang
    ENG
  • Data Source
    J-STAGE 
Page Top