Genetic Analyses of Fancy Rat-Derived Mutations

• KURAMOTO Takashi

  Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University

• YOKOE Mayuko

  Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University

• YAGASAKI Kayoko

  Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University

• KAWAGUCHI Tatsuya

  Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University

• KUMAFUJI Kenta

  Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University

• SERIKAWA Tadao

  Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University

抄録

To collect rat mutations and increase the value of the rat model system, we introduced fancy-derived mutations to the laboratory and carried out genetic analyses. Six fancy rats were shipped from a fancy rat colony in the USA and used as founders. After initial crosses with a laboratory strain, TM/Kyo or PVG/Seac, inbreeding started and 6 partially inbred lines, including 2 sublines, were produced as Kyoto Fancy Rat Stock (KFRS) strains. During inbreeding, we isolated 9 mutations: 5 coat colors, American mink (am), Black eye (Be), grey (g), Pearl (Pel), siamese (sia); 1 coat pattern, head spot (hs); 2 coat textures, Rex (Re), satin (sat); and an ear pinnae malformation, dumbo (dmbo). Genetic analyses mapped 7 mutations to particular regions of the rat chromosomes (Chr): am to Chr 1, sia to Chr 1, sat to Chr 3, Re to Chr 7, g to Chr 8, dmbo to Chr 14, and hs to Chr 15. Candidate gene analysis revealed that a missense mutation in the tyrosinase gene, Ser79Pro, was responsible for sia. From mutant phenotypes and mapping positions, it is likely that all mutations isolated in this study were unique to the fancy rat. These findings suggest that fancy rat colonies are a good source for collecting rat mutations. The fancy-derived mutations, made available to biomedical research in the current study, will increase the scientific value of laboratory rats.<br>

収録刊行物

• Experimental Animals

  Experimental Animals 59 (2), 147-155, 2010

  公益社団法人 日本実験動物学会

キーワード

• bioresource
• coat color
• genetic mapping
• inbreeding
• mutation

詳細情報

• CRID: 1390001205045847424
• NII論文ID: 130000254342
• NII書誌ID: AA11032321
• DOI: 10.1538/expanim.59.147
• ISSN: 18817122
• ISSN: 00075124
• ISSN: 13411357
• NDL書誌ID: 10811250
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10811250
• Web Site: http://www.jstage.jst.go.jp/article/expanim/59/2/59_2_147/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2010293329
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可