中性子と放射光の相補的な利用による創薬標的タンパク質の立体構造解析

DOI Web Site Web Site 被引用文献1件 参考文献13件

書誌事項

タイトル別名
  • Collaborative Use of Neutron and X-ray for Determination of Drug Target Proteins
  • チュウセイシ ト ホウシャコウ ノ ソウホテキ ナ リヨウ ニ ヨル ソウヤク ヒョウテキ タンパクシツ ノ リッタイ コウゾウ カイセキ

この論文をさがす

抄録

  Crystallography enables us to obtain accurate atomic positions within proteins. High resolution X-ray crystallography provides information for most of the atoms comprising a protein, with the exception of hydrogens. Neutron diffraction data can provide information of the location of hydrogen atoms, and is complementary to the structural information determined by X-ray crystallography. Here, we show the recent result of the structural determination of drug-target proteins, porcine pancreatic elastase and human immuno-deficiency virus type-1 protease by both X-ray and neutron diffraction. The structure of porcine pancreatic elastase with its potent inhibitor was determined to 0.94 Å resolution by X-ray diffraction and 1.65 Å resolution by neutron diffraction. The structure of HIV-PR with its potent inhibitor was also determined to 0.93 Å resolution by X-ray diffraction and 1.9 Å resolution by neutron diffraction. The ionization state and the location of hydrogen atoms of the catalytic residue in these enzymes were determined by neutron diffraction. Furthermore, collaborative use of both X-ray and neutron to identify the location of ambiguous hydrogen atoms will be shown.<br>

収録刊行物

  • 薬学雑誌

    薬学雑誌 130 (5), 657-664, 2010-05-01

    公益社団法人 日本薬学会

被引用文献 (1)*注記

もっと見る

参考文献 (13)*注記

もっと見る

関連プロジェクト

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ