Identification of Coagulation Inhibitor Proteins from Microcystis aeruginosa

• ISHIFUJI Shingo

  Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University

• SATO Yuichi

  Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University

• IMAE Hirotaka

  Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University

• TAKAARA Tomoko

  Department of Civil Engineering, Fukushima National College of Technology

• SANO Daisuke

  Division of Environmental Engineering, Graduate School of Engineering, Hokkaido University

• MASAGO Yoshifumi

  Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University

• OMURA Tatsuo

  Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University

Bibliographic Information

Other Title

• Ｍｉｃｒｏｃｙｓｔｉｓ　ａｅｒｕｇｉｎｏｓａ由来凝集阻害誘因タンパク質の同定

Abstract

Excess growth of cyanobacteria in semiclosed water area caused by eutrophication induces coagulation inhibition in drinking water treatment processes. Thus, it is highly desirable to establish effective countermeasures against such coagulation inhibition by identifying the causative substances released by cyanobacteria. In this study, coagulation inhibitor proteins of Microcystis aeruginosa were screened using a phage display system and an aluminum-ion-immobilized affinity chromatography. From the results, algal proteins with a high level of metal-coordinating amino acids were identified as inhibitor proteins for the coagulation. One of the inhibitor proteins, a homologue of the metal sensor protein ArsR, showed strong inhibition of the coagulation of kaolin suspension with polyaluminum chloride (PAC). It was demonstrated that one of the mechanisms of the coagulation inhibition caused by M. aeruginosa is the inhibition of the cross-linking between aluminum clusters in PAC and suspended solids by the algal inhibitor protein.

Journal

• Journal of Japan Society on Water Environment

  Journal of Japan Society on Water Environment 33 (6), 73-79, 2010

  Japan Society on Water Environment

Keywords

• <i>Microcystis aeruginosa</i>
• coagulation inhibition
• phage display
• polyaluminum chloride
• protein

Details

• CRID: 1390001205080988544
• NII Article ID: 130000307312
• DOI: 10.2965/jswe.33.73
• ISSN: 18813690
• ISSN: 09168958
• Web Site: https://www.jstage.jst.go.jp/article/jswe/33/6/33_6_73/_pdf
• Text Lang: ja
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed