Antimicrobial Susceptibility and Methicillin Resistance in Staphylococcus pseudintermedius and Staphylococcus schleiferi subsp. coagulans Isolated from Dogs with Pyoderma in Japan

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Abstract

To understand species distribution, trends of antimicrobial susceptibility and prevalence of methicillin resistance in canine staphylococci in Japan, 190 coagulase-positive staphylococci (CoPS) were isolated from dogs with pyoderma in 2 Japanese veterinary referral hospitals. Using a multiplex polymerase chain reaction (M-PCR) method, two CoPS species were identified: 170 <i>Staphylococcus</i> <i>pseudintermedius</i> (89.5%) and 20 <i>S. schleiferi</i> subsp. <i>coagulans</i> isolates (10.5%). In these isolates, susceptibility to 7 antimicrobial agents was determined. Overall, the levels of susceptibility to cefalexin (CEX), amoxicillin/clavulanic acid (CVA/AMPC), minocycline (MINO), ofloxacin (OFLX), norfloxacin (NFLX), lincomycin (LCM) and clindamycin (CLDM) in <i>S. pseudintermedius</i> isolates were 38.2, 52.4, 34.7, 31.2, 34.1, 1.2 and 11.2%, respectively. In <i>S. schleiferi</i> subsp. <i>coagulans</i> isolates, 55% demonstrated susceptibility to CEX, 80% to CVA/AMPC, 70% to MINO, 45% to OFLX or NFLX and 30% to CLDM. None of <i>S. schleiferi</i> subsp. <i>coagulans</i> isolates was susceptible to LCM. To determine the prevalence of methicillin-resistant strains, we used a PCR method, which enabled detection of the fragment of <i>mecA</i> gene in 66.5% (113 of 170) in <i>S. pseudintermedius</i> and 30.0% (6 of 20) in <i>S. schleiferi</i> subsp. <i>coagulans</i> isolates. The frequencies of susceptibility to CEX, CVA/AMPC, OFLX, NFLX and CLDM were significantly lower in methicillin-resistant CoPS than in methicillin-susceptible CoPS isolates. These data suggest a high level of methicillin resistance in staphylococci isolated from dogs with pyoderma in Japan.<br>

Journal

  • Journal of Veterinary Medical Science

    Journal of Veterinary Medical Science 72(12), 1615-1619, 2010

    JAPANESE SOCIETY OF VETERINARY SCIENCE

Cited by:  1

Codes

  • NII Article ID (NAID)
    130000307697
  • NII NACSIS-CAT ID (NCID)
    AA10796138
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    0916-7250
  • NDL Article ID
    10954356
  • NDL Source Classification
    ZR22(科学技術--農林水産--畜産)
  • NDL Call No.
    Z18-350
  • Data Source
    CJPref  NDL  J-STAGE 
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